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Changes to microdomain lipid composition may contribute to altered structure and function of key regulatory processes; however, knowledge regarding changes to membrane lipid composition after HIE is incomplete. Here, we describe the application of untargeted lipidomics to investigate the impact of hypoxia\u2013ischemia on detergent\u2010resistant membrane (DRM) and detergent\u2010soluble membrane (DSM) domains fractionated from the cerebellum in a rat model of HIE. Lipidomics utilized ultra performance liquid chromatography coupled to data independent tandem mass spectrometry with traveling wave ion mobility. Lipid alterations specific to the DRM domains after hypoxia\u2013ischemia included lysophospholipids, sphingomyelins, ceramides, triglycerides, and phosphatidylethanolamines, which are lipid species that have been linked to cognitive and neuronal impairment. The advances in lipidomics have enabled the tools available to study lipid composition. These data may provide insight into membrane disruption after HIE in relation to lipid composition and concentration.<\/jats:p>","DOI":"10.1002\/jms.70000","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:31:02Z","timestamp":1767677462000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lipidomic Comparison of Detergent\u2010Soluble and Detergent\u2010Resistant Lipid Domains Isolated From Rat Cerebellum and the Effect of Hypoxia\u2013Ischemia Using UPLC\u2010HDMS\n                    <sup>E<\/sup>"],"prefix":"10.1002","volume":"61","author":[{"given":"Samuel\u00a0A.","family":"Krug","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Maryland School of Pharmacy  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"He","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ningfeng","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4809-2250","authenticated-orcid":false,"given":"Cynthia","family":"Bearer","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5525-9170","authenticated-orcid":false,"given":"Maureen\u00a0A.","family":"Kane","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Maryland School of Pharmacy  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R200021\u2010JLR200"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.01.009"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2012.01.007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2977"},{"key":"e_1_2_8_6_1","first-page":"4","article-title":"Membrane Domains and the \u201cLipid Raft\u201d Concept","volume":"20","author":"Sonnino S.","year":"2012","journal-title":"Current Medicinal Chemistry"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.08.022"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0908987106"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/09687688.2012.700490"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.16"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471\u20104159.2002.00884.x"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22615"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.14.1.111"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1752-5_5"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201200044"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00041433-200006000-00002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2020.101068"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2017.08.012"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201035202\u20108"},{"key":"e_1_2_8_20_1","first-page":"1107","article-title":"Severe Alterations in Lipid Composition of Frontal Cortex Lipid Rafts From Parkinson's Disease and Incidental Parkinson's Disease","volume":"100","author":"Fabelo N.","year":"2003","journal-title":"Molecular medicine (Cambridge, Mass.)"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200505047"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200901145"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2013.03.005"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0631608100"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565\u2010020\u201009573\u2010x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky948"},{"key":"e_1_2_8_27_1","unstructured":"S. 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