{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T16:06:26Z","timestamp":1773331586951,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The effectiveness of L\u2010asparaginase and therefore the effectiveness of acute lymphoblastic leukemia treatment will be understood by determining the substrates of the enzyme, L\u2010asparagine and L\u2010glutamine. For this purpose, the high\u2010performance liquid chromatography\u2013tandem mass spectrometry method was developed and validated by analyzing the L\u2010asparaginase substrates asparagine and glutamine and its products aspartic acid and glutamic acid from plasma. Acetonitrile and ammonium acetate were used at 0.4\u2009mL\/min in gradient mobile phase flow using a HILIC column for chromatographic separations. The linear amino acid range was found to be 500\u20135000\u2009ng\/mL for asparagine, aspartic acid, and glutamic acid, and 5\u201350\u2009\u03bcg\/mL for glutamine, respectively. Detection limit and quantitation limit were found to be 100\u2013500\u2009ng\/mL for asparagine, aspartic acid, and glutamic acid, and 1\u20135\u2009\u03bcg\/mL for glutamine, respectively. The validated method has been successfully applied to plasma samples. The method was found to be selective and reproducible.<\/jats:p>","DOI":"10.1002\/jms.70036","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T05:30:51Z","timestamp":1771047051000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["HPLC\u2010MS\/MS Method for Monitoring of L\u2010Asparaginase Activity by Using Asparagine and Aspartic Acid Plasma Levels"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6227-0709","authenticated-orcid":false,"given":"Cem","family":"Kaplan","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Department of Analytical Chemistry \u0130stanbul University  Istanbul Turkey"},{"name":"Institute of Health Science, Department of Analytical Chemistry \u0130stanbul University  Istanbul Turkey"},{"name":"Drug Application and Research Center \u0130stanbul University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4646-4129","authenticated-orcid":false,"given":"\u0130brahim","family":"Dani\u015f","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Analytical Chemistry \u0130stanbul University  Istanbul Turkey"},{"name":"Drug Application and Research Center \u0130stanbul University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duri\u015fehvar\u00a0\u00d6zer","family":"\u00dcnal","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Analytical Chemistry \u0130stanbul University  Istanbul Turkey"},{"name":"Drug Application and Research Center \u0130stanbul University  Istanbul Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"issue":"4","key":"e_1_2_9_2_1","first-page":"162","article-title":"Akut Lenfoblastik L\u00f6semili \u00c7ocuklarda L\u2010Asparajinaz Tedavisi ve Komplikasyonlar\u0131","volume":"39","author":"Akarsu S.","year":"2004","journal-title":"T\u00fcrk Pediatri Ar\u015fivi"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.19.02292"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2017.1386305"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.12793\/tcp.2018.26.3.134"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.27729"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(96)00131-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.137380"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00232-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1096"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4500"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00182-4"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12014"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmy053"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2009.01.068"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/es900988q"},{"issue":"2","key":"e_1_2_9_17_1","first-page":"131","article-title":"Validation of a Reversed\u2010Phase HPLC Method for Quantitative Amino Acid Analysis","volume":"17","author":"Bartolomeo M. 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