{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:32:44Z","timestamp":1792204364580,"version":"4.4.0"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Dynamic protein complexes are central to cellular function, but capturing their native architectures remains challenging. While native ion mobility spectrometry\u2013mass spectrometry (IMS\u2010MS) can characterize these assemblies, its broader adoption is limited by the need for robust and automated sample introduction platforms. Here, we integrate a sheath\u2010liquid capillary electrophoresis interface with traveling\u2010wave IMS\u2010MS (\n                    <jats:sc>CE<\/jats:sc>\n                    \u2010TWIMS\u2010MS) to interrogate intact protein assemblies across a wide range of infusion flow rates. Using streptavidin as a benchmark, we show that native\u2010like structures are preserved across infusion flows, in excellent agreement with previously reported TWIMS\u2010MS measurements. The\n                    <jats:sc>CE<\/jats:sc>\n                    \u2010TWIMS\u2010MS platform provides precise fluidic control, enabling a tunable balance between sensitivity and spectral resolution. Low infusion flows enhance detection of low charge state populations, whereas intermediate flows maximize overall ion signal intensity. Flow\u2010dependent effects influence ion transmission efficiency and peak definition rather than gas\u2010phase conformations. Collectively, these results demonstrate that\n                    <jats:sc>CE<\/jats:sc>\n                    \u2010TWIMS\u2010MS provides a robust platform for native analysis, enabling reproducible and flow\u2010tunable characterization of intact protein assemblies.\n                  <\/jats:p>","DOI":"10.1002\/jms.70075","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T09:15:09Z","timestamp":1781169309000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Sheath\u2010Liquid\n                    <scp>CE<\/scp>\n                    \u2010MS Interface: A Robust Infusion Platform for Native IMS\u2010MS Analysis of Protein Assemblies and Their Conformers"],"prefix":"10.1002","volume":"61","author":[{"given":"Kanchan","family":"Pathak","sequence":"first","affiliation":[{"name":"Department of Chemistry Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delaynie","family":"Brinkman","sequence":"additional","affiliation":[{"name":"Department of Chemistry Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio P.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Chemistry Virginia Commonwealth University  Richmond Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2016.11.023"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-025-01866-8"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180052"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b05481"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3022"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10490-9"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp206867a"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.12.017"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2025.1688455"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00600"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2012.11.013"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac202103p"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01945"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00212"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(98)00157-3"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(03)00128-4"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.1c00322"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac0613380"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b03856"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5MD00617A"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8AY02159G"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-013-7226-1"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.5c00074"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.3c00371"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-017-1781-1"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202306824"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c06099"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202408370"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c06949"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c03784"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202000317"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","unstructured":"G.Zhu Y.Yue J. A.Col\u00f3n Rosado G.Gao X.Liu andL.Sun \u201cDecades of Dreams Coming True: Capillary Zone Electrophoresis\u2010Mass Spectrometry for Reproducible Multi\u2010Level Proteomics \u201d bioRxiv (2026).","DOI":"10.64898\/2026.01.28.702308"},{"key":"e_1_2_10_34_1","doi-asserted-by":"crossref","unstructured":"K.Pathak D.Brinkman S.Walters G.Perrotti B.Fuglestad andF.Gomes \u201cPinpointing Non\u2010Covalent Lipid Binding Sites in Membrane Proteins via Native Top\u2010Down Mass Spectrometry \u201d (2025).","DOI":"10.26434\/chemrxiv-2025-bmf0q"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-anchem-071124-092242"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200800664"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21599"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1444"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.11.086"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c03557"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/1044-0305(95)00191-F"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-025-01229-6"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201308139"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2015.06.003"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b00100"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4672"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00140"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.78"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac1022953"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6211"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja305213z"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b05519"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.3c00320"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.70034"},{"key":"e_1_2_10_55_1","article-title":"T\u2010Wave Ion Mobility\u2010Mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis","volume":"41","author":"Michaelevski I.","year":"2010","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c01270"}],"container-title":["Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.70075","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jms.70075","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.70075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T03:22:15Z","timestamp":1783480935000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jms.70075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,11]]},"references-count":55,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/jms.70075"],"URL":"https:\/\/doi.org\/10.1002\/jms.70075","archive":["Portico"],"relation":{},"ISSN":["1076-5174","1096-9888"],"issn-type":[{"value":"1076-5174","type":"print"},{"value":"1096-9888","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,11]]},"assertion":[{"value":"2026-02-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70075"}}