{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T06:20:06Z","timestamp":1787898006809,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515140152"],"award-info":[{"award-number":["2024A1515140152"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011480","name":"Foshan University","doi-asserted-by":"publisher","award":["CGZ07001"],"award-info":[{"award-number":["CGZ07001"]}],"id":[{"id":"10.13039\/501100011480","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Tumor progression locus 2 (TPL2, also known as MAP\u20103K8\/COT) is a kinase that regulates MAPK and NF\u2010\u03baB signaling and contributes to antiviral immunity. While mammalian TPL2 has been widely studied, the TPL2\u2010associated protein profile in swine cells remains largely unexplored. In this study, we generated an IP\u2013LC\u2013MS\/MS\u2010based candidate dataset of TPL2\u2010associated proteins in porcine kidney epithelial (PK\u201015) cells. FLAG\u2010tagged TPL2 was overexpressed in PK\u201015 cells and enriched by immunoprecipitation, followed by liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) analysis. After subtraction of proteins detected in the empty\u2010vector negative IP control, a total of 666 candidate TPL2\u2010associated proteins were identified. Bioinformatic analyses, including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein\u2013protein interaction (PPI) network analysis, indicated that these candidates were mainly associated with RNA binding, protein modification, ubiquitin\u2013proteasome processes, spliceosome\u2010related pathways, and immune\u2010associated signaling. Collectively, our results provide an IP\u2013LC\u2013MS\/MS\u2010based candidate resource of TPL2\u2010associated proteins in porcine PK\u201015 cells, offering a useful basis for future studies on TPL2\u2010mediated host signaling and virus\u2013host interactions.<\/jats:p>","DOI":"10.1002\/jms.70086","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T10:17:50Z","timestamp":1784024270000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["IP\u2013LC\u2013MS\/MS\u2010Based Identification of Candidate TPL2 (MAP3K8)\u2010Associated Proteins in Porcine PK\u201015 Cells"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-5245-7168","authenticated-orcid":false,"given":"Dajun","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Animal Science and Technology Foshan University  Foshan 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