{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:46:21Z","timestamp":1791989181534,"version":"4.4.0"},"reference-count":53,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Today, matrix\u2010assisted laser desorption\/ionization\u2010intact cell mass spectrometry (MALDI\u2010ICMS) is used in clinical environments for rapid pathogen identification, referred to as MALDI biotyping. This method relies on the generation of characteristic mass spectrometric profiles of intact microorganisms. Single\u2010celled organisms are often used for pharmaceutical production, while this allows for the production of complex products, close monitoring and control of these processes is essential. In this study, we propose the use of MALDI\u2010ICMS as a potential at\u2010line approach for fast monitoring of the progress of a biotechnologically relevant fermentation process employing\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    as a host. We demonstrate the reproducibility of the method, thereby indicating its potential to predict the progress of antibody fragment production in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    in an industrial setting. We evaluated various aspects of sample preparation, including the choice of matrix and solvent concentration, sample loading, and sample\u2010to\u2010matrix ratios. Our workflow allowed monitoring of over 100 signals across a 32\u2010h span of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    fermentation. To control instrumental variability, we performed eight technical replicates and achieved excellent repeatability, with 9% median relative standard deviation of peak intensities. Principal component analysis revealed strong time\u2010related differences of the mass spectra, allowing to differentiate the data based on fermentation phases. Furthermore, to assess direct monitoring of product expression, we adapted acquisition parameters to enable product detection via MALDI\u2010ICMS. The presented MALDI\u2010ICMS workflow allowed reliable monitoring of cellular changes during fermentation, thereby expanding the applications of MALDI\u2010ICMS to process analytical technologies (PAT).\n                  <\/jats:p>","DOI":"10.1002\/jms.70110","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:03:07Z","timestamp":1791986587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Validation of a MALDI Intact Cell Mass Spectrometry Workflow for Routine Application in Monitoring Industrial\n                    <scp>\n                      <i>E. coli<\/i>\n                    <\/scp>\n                    Fermentations"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5019-2408","authenticated-orcid":false,"given":"Cristian","family":"Zanetti","sequence":"first","affiliation":[{"name":"Institute of Chemical Technologies and Analytics TU Wien  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Stephan","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim RCV GmbH &amp; Co KG  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Bednar","sequence":"additional","affiliation":[{"name":"Institute of Chemical Technologies and Analytics TU Wien  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Foettinger\u2010Vacha","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim RCV GmbH &amp; Co KG  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8060-7851","authenticated-orcid":false,"given":"Martina","family":"Marchetti\u2010Deschmann","sequence":"additional","affiliation":[{"name":"Institute of Chemical Technologies and Analytics TU Wien  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290081224"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1196\u20101584"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20100231(19960731)10:10<>3.0.CO;2\u20106"},{"key":"e_1_2_8_5_1","first-page":"197","volume-title":"Advances in MALDI and Laser\u2010Induced Soft Ionization Mass Spectrometry","author":"Pranada A. 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