{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:48:54Z","timestamp":1791679734935,"version":"4.3.4"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Nicotiana glauca<\/jats:italic>\n                    <\/jats:styled-content>\n                    Graham (Solanaceae), also known as tree tobacco, naturally grows in dry, arid regions of North America, Africa, and Europe. It is recognized for both toxic and medicinal properties. This study aimed to analyze the metabolic profile of crude leaf extracts from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. glauca<\/jats:italic>\n                    <\/jats:styled-content>\n                    using liquid chromatography\u2013electrospray ionization tandem mass spectrometry (LC\u2010ESI\u2010MS\/MS). A total of 16 different compounds were identified, including five alkaloids, two coumarins, two flavonoids, and two monoterpenes, as well as four hydroxycinnamic acid derivatives and one homoserine lactone. Some of these compounds were reported for the first time in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. glauca<\/jats:italic>\n                    <\/jats:styled-content>\n                    . According to the available literature, this is the first study to report the presence of puromycin in the genus\n                    <jats:italic>Nicotiana<\/jats:italic>\n                    . In addition, molecular docking studies were carried out to investigate the active site of human peroxiredoxin 5 (PDB ID: 1HD2), aiming to elucidate the potential interactions between the identified compounds and this receptor. These results suggest that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. glauca<\/jats:italic>\n                    <\/jats:styled-content>\n                    could be a good source of natural chemicals for future medical or nutritional research.\n                  <\/jats:p>","DOI":"10.1002\/jms.70111","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T06:30:17Z","timestamp":1789367417000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phytochemical Identification of Major Compounds From Leaves of\n                    <i>Nicotiana glauca<\/i>\n                    Graham (Solanaceae) Extracts Using LC\u2010ESI\u2010MS\/MS and Molecular Docking Analysis"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0268-1383","authenticated-orcid":false,"family":"WardaTrifa","sequence":"first","affiliation":[{"name":"Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry University Mentouri Constantine 1  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4999-5673","authenticated-orcid":false,"given":"Salah","family":"Akkal","sequence":"additional","affiliation":[{"name":"Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry University Mentouri Constantine 1  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Perruchon","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNIROUEN, INSA  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0665-0074","authenticated-orcid":false,"given":"Isabelle","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNIROUEN, INSA  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2406-5664","authenticated-orcid":false,"given":"Carlos","family":"Afonso","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNIROUEN, INSA  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelhakim","family":"Elomri","sequence":"additional","affiliation":[{"name":"Normandie Univ, UNIROUEN, INSA  Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0943-5214","authenticated-orcid":false,"given":"Aziza","family":"Huseynova","sequence":"additional","affiliation":[{"name":"Faculty of Medical Nakhchivan State University  Nakhchivan Azerbaijan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2564-8343","authenticated-orcid":false,"given":"Meral","family":"Keke\u00e7o\u011flu","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Biology, Beekeeping Research Development and Application Centre Duzce University  Duzce Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3742-8727","authenticated-orcid":false,"given":"Rafik","family":"Menacer","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Sciences Pharmaceutiques (CRSP)  Constantine Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3989\/gya.0927182"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2020.12.034"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0379-0738(02)00123-8"},{"key":"e_1_2_8_5_1","first-page":"294","article-title":"Antiproliferative Activity of Three Wild Growing Species in Tunisia: Nicotianaglauca, Artemisia Campestris and Astragalus gombo","volume":"3","author":"Teyeb H.","year":"2017","journal-title":"International Journal of Pharmacognosy"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.5289"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2478\/cipms-2020-0007"},{"key":"e_1_2_8_8_1","first-page":"3","article-title":"Nicotine and Tobacco Alkaloids: A GC\u2010MS Approach","volume":"2","author":"Zbancioc G.","year":"2012","journal-title":"International Journal of Criminal Investigation"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7758"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42777-8"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25092040"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2478\/acph-2022-0001"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25020357"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmaa061"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.009431"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.5935\/0100-4042.20140069"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2017.01.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.02.001"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2004.02.009"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083647"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874088X01812010058"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-020-03040-6"},{"key":"e_1_2_8_23_1","first-page":"315","article-title":"Determination of the Antioxidant Activity of Rutin and Its Contribution to the Antioxidant Capacity of Diversified Buckwheat Origin Material by Updated Analytical Strategies","volume":"60","author":"Zieli\u0144ska D.","year":"2010","journal-title":"Polish Journal of Food And Nutrition Sciences"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2016.04.025"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2017.01.023"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02974002"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.4236\/fns.2011.230025"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.576319"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4FO00042K"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.05.080"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-4-17"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4853"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi0814s24"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-2269-7_19"},{"key":"e_1_2_8_35_1","first-page":"240","article-title":"Discovery Studio Visualizer","volume":"936","author":"Biovia D. S.","year":"2017","journal-title":"San Diego, CA, USA"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty707"}],"container-title":["Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.70111","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jms.70111","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.70111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:17:06Z","timestamp":1791677826000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jms.70111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,13]]},"references-count":35,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/jms.70111"],"URL":"https:\/\/doi.org\/10.1002\/jms.70111","archive":["Portico"],"relation":{},"ISSN":["1076-5174","1096-9888"],"issn-type":[{"value":"1076-5174","type":"print"},{"value":"1096-9888","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,13]]},"assertion":[{"value":"2026-07-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70111"}}