{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:49:51Z","timestamp":1791874191989,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Mass Spectrom"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Screening of major cannabinoids in cannabis\u2010derived extracts is needed when these extracts are applied in pharmacological and biological studies. Precursor ion scan\u2010mass spectrometry (PIS\u2010MS) is a viable approach for the detection and screening of major cannabinoids through characteristic MS\/MS fragmentation behavior. A PIS\u2010MS method was developed utilizing electrospray ionization (ESI) operated in the positive ion mode on a triple quadrupole\u2010linear ion trap mass spectrometer (QTRAP 6500). Three cannabis extracts were screened based on established MS\/MS fragmentation pattern. Characteristic product ions at\n                    <jats:italic>m\/z<\/jats:italic>\n                    193.1 and 233.1 were optimized for PIS\u2010MS analysis. In addition, tandem mass spectrometry (MS\/MS) was performed to aid in the putative identification of unknown cannabinoids, whereas authentic standards were used, when available, to confirm the identity. A quadrupole\u2010Orbitrap (Exploris 240) mass spectrometer equipped with ESI was also utilized to provide accurate mass measurements for single\u2010stage and MS\/MS analyses, supporting the proposed structures of cannabinoids and their product ions. PIS\u2010MS complemented with high\u2010resolution mass spectrometry (HRMS) analysis revealed the existence of six major cannabinoids in two plant extracts, and nine in the third one. Among the detected phytocannabinoids, an unexpected cannabinoid, namely, sesquicannabigerol (Sesqui\u2010CBG), was also identified among the most abundant ones. The MS\/MS fragmentation behavior in both positive and negative ionization for Sesqui\u2010CBG was established for the first time, supported by accurate mass measurements and second\u2010generation MS\/MS (MS\n                    <jats:sup>3<\/jats:sup>\n                    ) experiments. In conclusion, PIS\u2010MS provided an effective approach for the screening of abundant cannabinoids in plant products intended for therapeutic applications. MS\/MS and accurate mass measurements confirmed the putative identification of detected cannabinoids. A comprehensive study for Sesqui\u2010CBG was performed, elucidating its fragmentation pathways and product ions.\n                  <\/jats:p>","DOI":"10.1002\/jms.70119","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:17:07Z","timestamp":1791872227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Development of a Simple Liquid Chromatography\u2010Precursor Ion Scan\u2010Mass Spectrometry Screening Method for the Detection of Phytocannabinoids in Plant Extracts"],"prefix":"10.1002","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0130-4594","authenticated-orcid":false,"given":"Radwa","family":"Mahmoud","sequence":"first","affiliation":[{"name":"College of Pharmacy and Nutrition University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5161-8677","authenticated-orcid":false,"given":"Deborah","family":"Michel","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Nutrition University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Elessawy","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Nutrition University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1274-837X","authenticated-orcid":false,"given":"Randy W.","family":"Purves","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Nutrition University of Saskatchewan  Saskatoon Saskatchewan Canada"},{"name":"Centre for Veterinary Drug Residues Canadian Food Inspection Agency  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert B.","family":"Laprairie","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Nutrition University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1060-3609","authenticated-orcid":false,"given":"Anas","family":"El\u2010Aneed","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Nutrition University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.lfs.2005.09.011","article-title":"Chemical Constituents of Marijuana: The Complex Mixture of Natural Cannabinoids","volume":"78","author":"ElSohly M. 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