{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:02:02Z","timestamp":1761894122209,"version":"build-2065373602"},"reference-count":18,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T00:00:00Z","timestamp":1115856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Mass Spectrom."],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The novel and rapid assay presented here combines high\u2010performance liquid chromatography and electrospray ionisation tandem mass spectrometry (HPLC\u2010ESI\u2010MS\/MS) to directly measure and quantify the CoA esters of 3\u03b1,7\u03b1,12\u03b1\u2010trihydroxy\u2010 and 3\u03b1,7\u03b1\u2010dihydroxy\u20105\u03b2\u2010cholestan\u201026\u2010oic acid (THCA and DHCA). The latter are converted inside peroxisomes to the primary bile acids, cholic and chenodeoxycholic acids, respectively. Prior to MS\/MS, esters were separated by reversed\u2010phase HPLC on a C<jats:sub>18<\/jats:sub> column using an isocratic mobile phase (acetonitrile\/water\/2\u2010propanol) and subsequently detected by multiple reaction monitoring. For quantification, the CoA ester of deuterium\u2010labelled 3\u03b1,7\u03b1,12\u03b1\u2010trihydroxy\u20105\u03b2\u2010cholan\u201024\u2010oic acid (<jats:italic>d<\/jats:italic><jats:sub>4<\/jats:sub>\u2010CA) was used as internal standard. To complete an assay took less than 8 min.<\/jats:p><jats:p>To verify the validity of the assay, the effect of peroxisomal proteins on the efficacy of extraction of the CoA esters was tested. To this end, variable amounts of the CoA esters were spiked with a fixed amount of either intact peroxisomes or peroxisomal matrix proteins and then extracted using a solid\u2010phase extraction system. The CoA esters could be reproducibly recovered in the range of 0.1\u20134 \u00b5mol l<jats:sup>\u22121<\/jats:sup> (linear correlation coefficient R<jats:sup>2<\/jats:sup> &gt; 0.99), with a detection limit of 0.1 \u00b5mol l<jats:sup>\u22121<\/jats:sup>.<\/jats:p><jats:p>In summary, electrospray ionization tandem mass spectrometry combined with HPLC as described here proved to be a rapid and versatile technique for the determination of bile acid CoA esters in a mixture with peroxisomal proteins. This suggests this technique to become a valuable tool in studies dealing with the multi\u2010step biosynthesis of bile acids and its disturbances in disorders like the Zellweger syndrome. Copyright \u00a9 2005 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/jms.864","type":"journal-article","created":{"date-parts":[[2005,5,12]],"date-time":"2005-05-12T09:16:33Z","timestamp":1115889393000},"page":"882-889","source":"Crossref","is-referenced-by-count":11,"title":["Measurement of bile acid CoA esters by high\u2010performance liquid chromatography\u2013electrospray ionisation tandem mass spectrometry (HPLC\u2013ESI\u2010MS\/MS)"],"prefix":"10.1002","volume":"40","author":[{"given":"H.","family":"Gan\u2010Schreier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. G.","family":"Okun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Kohlmueller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.\u2010D.","family":"Langhans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Peters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. J.","family":"ten Brink","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. M.","family":"Verhoeven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Jakobs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Voelkl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. F.","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,12]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-0898-0_1"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7306(08)60685-7"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(80)80377-2"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(84)90341-2"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)37854-8"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112252"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00135a001"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1630181"},{"key":"e_1_2_5_10_2","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/S0022-2275(20)32342-7","article-title":"Determination of bile acid in biological fluids by liquid chromatography\u2010electrospray tandem mass spectrometry","volume":"42","author":"Perwaiz S","year":"2001","journal-title":"J. Lipid Res."},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2001.5379"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199803000-00009"},{"key":"e_1_2_5_13_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0022-2275(20)32330-0","article-title":"Rapid and quantitative analysis of unconjugated C27 bile acids in plasma and blood samples by tandem mass spectrometry","volume":"42","author":"Johnson DW","year":"2001","journal-title":"J. Lipid Res."},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(97)00601-6"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.14.070194.002015"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0890296"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb08920.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199509000-00016"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365529409103629"}],"container-title":["Journal of Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjms.864","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jms.864","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:13:55Z","timestamp":1760631235000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jms.864"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,5,12]]},"references-count":18,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1002\/jms.864"],"URL":"https:\/\/doi.org\/10.1002\/jms.864","archive":["Portico"],"relation":{},"ISSN":["1076-5174","1096-9888"],"issn-type":[{"type":"print","value":"1076-5174"},{"type":"electronic","value":"1096-9888"}],"subject":[],"published":{"date-parts":[[2005,5,12]]}}}