{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T22:51:26Z","timestamp":1768517486109,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,4,2]],"date-time":"2001-04-02T00:00:00Z","timestamp":986169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Approximately 20 cases of tick\u2010borne encephalitis (TBE) occur annually in Finland. The known endemic areas are situated mainly in the archipelago and coastal regions of Finland, with highest incidence in \u00c5land islands. <jats:italic>Ixodes ricinus<\/jats:italic> panels collected in 1996\u20131997 from two endemic areas were screened for the presence of RNA. Two distinct RT\u2010PCR methods were applied, and were shown to have an approximate detection limit of 10 focus forming doses (FFD)\/100 \u03bcl. One out of 20 pools (a total of 139 ticks) from Helsinki Isosaari Island and one out of 48 pools (a total of 450 ticks) from \u00c5land were positive with both methods, whereas the remaining pools were negative. The observed overall frequency (0.34%) in ticks in endemic areas of Finland, was similar to the low incidence found by virus isolation in mice in the 1960s (0.5%). Viral RNA was detectable in a diluted sample representing 0.005% of a positive pool of ten nymphs suggesting that the viral RNA load within an infected tick pool was approximately equivalent to 20,000\u2013200,000 FFD. Sequence analysis did not show geographical clustering of the Finnish strains, suggesting an independent emergence of different TBE foci from the south. TBE virus RNA positive ticks were not found in <jats:italic>I. ricinus<\/jats:italic> panels consisting of 130 pools (726 ticks) from Helsinki city parks or 41 pools (197 ticks) from V\u00f5rmsi Island in Estonia. J. Med. Virol. 64:21\u201328, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1012","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:27:27Z","timestamp":1030310847000},"page":"21-28","source":"Crossref","is-referenced-by-count":47,"title":["Prevalence of tick\u2010borne encephalitis virus in <i>Ixodes ricinus<\/i> ticks in 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