{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:09:57Z","timestamp":1791202197144,"version":"4.2.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,10,10]],"date-time":"2002-10-10T00:00:00Z","timestamp":1034208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["SFB466"],"award-info":[{"award-number":["SFB466"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"name":"USPHS-NIH","award":["PO1-DE-07946"],"award-info":[{"award-number":["PO1-DE-07946"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Deoxyuridine triphosphatase (dUTPase) catalyses the hydrolysis of dUTP to dUMP and pyrophosphate thus preventing the incorporation of uracil into replicating DNA. Previous studies of several virus models have suggested that viral dUTPases may be required for virus replication in resting cells whereas in proliferating cells cellular dUTPase may substitute for a mutant viral protein. Using monoclonal antibodies and immunohistochemistry, Epstein\u2010Barr virus\u2010associated non\u2010neoplastic and neoplastic diseases were studied for the expression of viral and human dUTPases. Oral hairy leukoplakia, an AIDS\u2010associated lesion of the tongue, is known to support EBV replication in the upper epithelial cell layers. In agreement with this, strong focal expression of EBV dUTPase was detected in the upper epithelial cell layers of oral hairy leukoplakia whereas expression of human dUTPase was confined to the basal proliferative cell compartment. Furthermore, in infectious mononucleosis tonsils, rare scattered small lymphoid cells expressed EBV dUTPase, consistent with the expression pattern of other EBV lytic cycle antigens. These findings are in agreement with the notion that EBV replicates in resting cells. Three EBV\u2010associated tumours, Hodgkin lymphoma, Burkitt lymphoma and nasopharyngeal carcinoma, lacked detectable expression of EBV dUTPase, in agreement with the notion that EBV infection is largely latent in these tumours. By contrast, expression of human dUTPase was observed regularly in these tumours. These results suggest that EBV dUTPase may be a suitable target for anti\u2010viral therapy and that inhibitors of human dUTPase should prove useful for the treatment of human tumours, including EBV\u2010associated cancers. J. Med. Virol. 68:568\u2013573, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.10234","type":"journal-article","created":{"date-parts":[[2002,10,11]],"date-time":"2002-10-11T22:29:47Z","timestamp":1034375387000},"page":"568-573","source":"Crossref","is-referenced-by-count":18,"title":["Expression of viral and human dUTPase in Epstein\u2010Barr virus\u2010associated diseases"],"prefix":"10.1002","volume":"68","author":[{"given":"Johann","family":"Fleischmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Kremmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S.","family":"Greenspan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich A.","family":"Gr\u00e4sser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Niedobitek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,10,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V85.3.744.bloodjournal853744"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.867"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01575-0"},{"key":"e_1_2_1_5_1","first-page":"5219","article-title":"Resistance to fluorodeoxyxuridine\u2010induced DNA damage and cytotoxicity correlates with an elevation of deoxyuridine triphosphatase activity and failure to accumulate deoxyuridine triphosphate","volume":"53","author":"Canman CE","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_1_6_1","first-page":"2296","article-title":"Induction of resistance to fluorodeoxyuridine cytotoxicity and DNA damage in human tumour cells by expression of Eshcerischia coli deoxyuridinetriphosphatase","volume":"54","author":"Canman CE","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(86)90414-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19991222)84:6<614::AID-IJC13>3.0.CO;2-P"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910310104"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198512193132502"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1128\/jvi.70.2.1242-1245.1996","article-title":"Identification and characterization of pseudorabies virus dUTPase","volume":"70","author":"J\u00f6ns A","year":"1996","journal-title":"J Virol"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00716184"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90192-5"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203013380964"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.18388\/abp.1997_4410","article-title":"dUTP pyrophosphatase as a potential target for chemotherapeutic drug development","volume":"44","author":"McIntosh EM","year":"1997","journal-title":"Acta Biochim Pol"},{"key":"e_1_2_1_16_1","first-page":"1031","article-title":"A new lytic antibody, 7D6, detects Epstein\u2010Barr virus dUTPase in nonkeratinizing undifferentiated nasopharyngeal carcinomas","volume":"78","author":"Nicholls JM","year":"1998","journal-title":"Lab Invest"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-72-12-3035"},{"key":"e_1_2_1_18_1","first-page":"879","article-title":"Expression of Epstein\u2010Barr virus genes and of lymphocyte activation molecules in undifferentiated nasopharyngeal carcinomas","volume":"140","author":"Niedobitek G","year":"1992","journal-title":"Am J Pathol"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.2.659.bloodjournal862659"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199706)182:2<151::AID-PATH824>3.0.CO;2-3"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.8934-8943.1999"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V78.5.1162.1162"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"6706","DOI":"10.1128\/jvi.66.11.6706-6713.1992","article-title":"Herpes simplex virus type 1 dUTPase mutants are attenuated for neurovirulence, neuroinvasiveness and reactivation from latency","volume":"66","author":"Pyles RB","year":"1992","journal-title":"J Virol"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1992.tb01008.x"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-11-2795"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1347"},{"key":"e_1_2_1_27_1","first-page":"1369","article-title":"Epstein\u2010Barr virus gene expression and epithelial cell differentiation in oral hairy leukoplakia","volume":"139","author":"Thomas JA","year":"1991","journal-title":"Am J Pathol"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.2.1213-1217.1996"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90171-7"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-5-1051"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.10234","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.10234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T08:00:15Z","timestamp":1700294415000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.10234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10,10]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1002\/jmv.10234"],"URL":"https:\/\/doi.org\/10.1002\/jmv.10234","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10,10]]}}}