{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:49:18Z","timestamp":1777502958440,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,1,13]],"date-time":"2003-01-13T00:00:00Z","timestamp":1042416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>EBV infects most of the global population, but only a small percentage of infected individuals develop EBV\u2010associated malignancies. Host and viral factors may play a role in determining the clinical outcome. Because EBNA\u20101 functions as an oriP binding protein and links the episomal viral DNA to metaphase chromosomes, variation of EBNA\u20101 has been suggested to contribute to determining the tissue tropism of EBV and the development of various EBV\u2010associated diseases. Five subtypes have been described, according to the amino acid at residue 487: P\u2010ala (B95\u20108 prototype), P\u2010thr (aa 487 ala to thr), V\u2010val, V\u2010pro and V\u2010leu. Other studies, however, concluded that EBNA\u20101 sequence variation simply reflects the geographical distribution of EBV. To clarify these possibilities, we collected DNA samples from healthy individuals and patients with various EBV\u2010associated diseases in Taiwan for PCR amplification and DNA sequencing. The results indicate that: 1) V\u2010val EBNA\u20101 was detected in patients with nasopharyngeal carcinoma (NPC) and other EBV\u2010associated malignant diseases; 2) the prototype P\u2010ala strain was detected only in peripheral blood lymphocytes; 3) mixed populations of different subtypes of N\u2010terminal and C\u2010terminal sequences were observed in samples from one patient with nasopharyngeal carcinoma, one with T lymphoma and one with infectious mononucleosis sample; 4) intermediate variations between P\u2010ala and V\u2010val were observed in T\u2010lymphoma, Hodgkin disease and infectious mononucleosis samples; and 5) in comparison with the major sequences identified in healthy carriers, the EBNA\u20101 sequences in peripheral lymphocytes from nasopharyngeal carcinoma were mixed types in 4 of 5 patients, implying increasing frequency of V\u2010val might correlate with the progression of nasopharyngeal carcinoma. J. Med. Virol. 69:417\u2013425, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.10305","type":"journal-article","created":{"date-parts":[[2003,1,14]],"date-time":"2003-01-14T21:04:39Z","timestamp":1042578279000},"page":"417-425","source":"Crossref","is-referenced-by-count":19,"title":["EBNA\u20101 sequence variations reflect active EBV replication and disease status or quiescentlatency in 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