{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T16:37:14Z","timestamp":1772728634061,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,4,9]],"date-time":"2003-04-09T00:00:00Z","timestamp":1049846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Eliminating Epstein\u2010Barr virus (EBV) genomes from infected cells is an intriguing theoretical strategy in therapy for EBV\u2010associated malignant diseases. Respective patterns were characterized for hydroxyurea (HU)\u2010promoted loss of EBV genomes from EBV\u2010infected epithelioid cell lines derived from the noncancerous portion of gastric carcinoma tissues and Burkitt's lymphoma (BL) cell lines. Epithelioid cell lines GT38 and PN were less sensitivity to HU than BL cell lines Akata, Raji, and Daudi in terms of cell growth inhibition and cell cycle arrest. On passage in medium with 50 \u03bcM HU, the fraction of EBV nuclear antigen (EBNA)\u2010positive cells was reduced substantially in the BL cell lines, but only slightly in the epithelioid cell lines. EBV DNA was reduced in Akata, Raji, and Daudi cells upon passage in 50 \u03bcM HU by 95%, 70%, and 50%, respectively, but by only 10% in GT38 cells, in which EBV DNA reduction was enhanced at increased concentrations of HU. This indicates that EBV genome is more easily lost from BL cell lines than from epithelioid cell lines upon culturing in HU. These findings support the view that the elimination of EBV could be therapeutically effective in EBV\u2010associated BL by HU. J. Med. Virol. 70: 244\u2013252, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.10384","type":"journal-article","created":{"date-parts":[[2003,4,10]],"date-time":"2003-04-10T10:01:57Z","timestamp":1049968917000},"page":"244-252","source":"Crossref","is-referenced-by-count":10,"title":["Contrasting effects of hydroxyurea on cell growth and reduction in Epstein\u2010Barr virus genomes in EBV\u2010infected epithelioid cell lines vs. Burkitt's lymphoma cell lines"],"prefix":"10.1002","volume":"70","author":[{"given":"Ru","family":"Jiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikiko","family":"Kanamori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Fukuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazufumi","family":"Ikuta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masanao","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Sairenji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,4,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.64.5.2369-2379.1990"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/515290"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1128\/jvi.34.2.560-568.1980","article-title":"Effect of acyclovir [9\u2010(hydroxyethoxymethyl)guanine] on Epstein\u2010Barr virus DNA replication","volume":"34","author":"Colby BM","year":"1980","journal-title":"J Virol"},{"key":"e_1_2_1_5_1","first-page":"11","article-title":"An overview of the clinical experience with hydroxyurea","volume":"19","author":"Donehower RC","year":"1992","journal-title":"Semin Oncol"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1071"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.19.8925"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.9748"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1166"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1128\/jvi.52.2.549-556.1984","article-title":"Electron microscopic evidence for replication of circular Epstein\u2010Barr virus genomes in latently infected Raji cells","volume":"52","author":"Gussander E","year":"1984","journal-title":"J Virol"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.3.631"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-56515-1_2"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.53.4.248"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.19.9131"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008110312661"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01468-X"},{"key":"e_1_2_1_17_1","first-page":"1300","article-title":"Surface IgM kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines","volume":"28","author":"Klein E","year":"1968","journal-title":"Cancer Res"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"9150","DOI":"10.1128\/JVI.72.11.9150-9156.1998","article-title":"Epstein\u2010Barr virus contributes to the malignant phenotype and to apoptosis resistance in Burkitt's lymphoma cell line Akata","volume":"72","author":"Komano J","year":"1998","journal-title":"J Virol"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.1.232-242.2002"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1086\/513974"},{"key":"e_1_2_1_21_1","first-page":"115","volume-title":"Inhibitors of ribonucleotide diphosphate reductase activity","author":"Moore EC","year":"1989"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0602"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.5.954"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199509143331103"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.18.3.261"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90803-2"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910150406"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-56515-1_12"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008021402908"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020113906966"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.68.9.6069-6073.1994"},{"key":"e_1_2_1_32_1","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1128\/jvi.53.1.299-301.1985","article-title":"Epstein\u2010Barr virus transformation of human B lymphocyte despite inhibition of viral polymerase","volume":"53","author":"Sixbey JW","year":"1985","journal-title":"J Virol"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02879-8"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1998.tb00557.x"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.63.1.445-449.1989"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9231"},{"key":"e_1_2_1_37_1","first-page":"1250","article-title":"Epstein\u2010Barr virus gastric carcinoma","volume":"143","author":"Tokunaga M","year":"1993","journal-title":"Am J Pathol"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.10.4929-4935.2001"},{"key":"e_1_2_1_39_1","first-page":"6273","article-title":"Hydroxyurea accelerates loss of extrachromosomally amplified genes from tumor cells","volume":"51","author":"Von Hoff DD","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.17.8165"},{"key":"e_1_2_1_41_1","first-page":"1","article-title":"Mechanisms of action of hydroxyurea","volume":"19","author":"Yarbro JW","year":"1992","journal-title":"Semin Oncol"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.10384","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.10384","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T02:58:22Z","timestamp":1700276302000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.10384"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4,9]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["10.1002\/jmv.10384"],"URL":"https:\/\/doi.org\/10.1002\/jmv.10384","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4,9]]}}}