{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:27:20Z","timestamp":1769603240940,"version":"3.49.0"},"reference-count":63,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,5,6]],"date-time":"2003-05-06T00:00:00Z","timestamp":1052179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Expression of different panels of latent gene transcripts is controlled by usage of three distinct Epstein\u2010Barr virus (EBV) nuclear antigen (EBNA) promoters (Wp, Cp, and Qp). EBV\u2010associated hemophagocytic syndrome, which is often a fatal disease and generally occurs after primary EBV infection, is characterized by monoclonal or oligoclonal proliferation of EBV\u2010infected T cells. The latency pattern and EBNA promoter (Wp, Cp, and Qp) usage in EBV\u2010infected cells from three patients with EBV\u2010associated hemophagocytic syndrome were examined by reverse transcription\u2010polymerase chain reaction (PCR). Three samples from the patients expressed EBER, EBNA1, EBNA2, latent membrane protein (LMP)1, and LMP2A transcripts. The transcripts of EBNA1 were initiated from not only Wp\/Cp but also Qp. Lytic cycle Fp\u2010initiated EBNA1 and EBV lytic gene BZLF1 transcripts were not detected. The methylation statuses of three EBNA promoters in three patients with EBV\u2010associated hemophagocytic syndrome and in two patients with infectious mononucleosis were also analyzed using bisulfite PCR analysis. Wp was hypermethylated, and Qp was unmethylated in both diseases. Cp was highly methylated in EBV\u2010associated hemophagocytic syndrome, however, whereas Cp was almost unmethylated in infectious mononucleosis. These results suggest that there may be distinct EBV\u2010infected cell populations in EBV\u2010associated hemophagocytic syndrome, which exhibit different patterns of EBV latent gene expression. The methylation status in Cp and phenotype of EBV\u2010infected cells may be critical differences in EBV\u2010associated hemophagocytic syndrome and infectious mononucleosis. J. Med. Virol. 70:410\u2013419, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.10411","type":"journal-article","created":{"date-parts":[[2003,5,21]],"date-time":"2003-05-21T10:02:00Z","timestamp":1053511320000},"page":"410-419","source":"Crossref","is-referenced-by-count":20,"title":["Latency pattern of Epstein\u2010Barr virus and methylation status in Epstein\u2010Barr virus\u2010associated hemophagocytic 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