{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:00:17Z","timestamp":1790078417484,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,6,2]],"date-time":"2003-06-02T00:00:00Z","timestamp":1054512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mitochondrial DNA (mtDNA) of peripheral blood mononuclear cells (PBMCs) collected from Human immunodeficiency virus 1 (HIV\u20101)\u2010infected patients and healthy controls were measured longitudinally using real\u2010time polymerase chain reaction to evaluate the effects of antiretroviral agents on mtDNA synthesis in vivo and to assess the value of monitoring mtDNA in PBMCs to predict adverse events amongst these patients. MtDNA levels in PBMCs were significantly decreased in treatment\u2010naive HIV\u20101\u2010infected patients compared with healthy people. MtDNA levels were not only significantly correlated with CD4<jats:sup>+<\/jats:sup> T\u2010cell count, but also inversely correlated with HIV\u20101 viral load. MtDNA levels in untreated patients and healthy controls were stable during the period of observation. On the other hand, amongst patients treated with regimens containing AZT\/3TC or d4T\/3TC, mtDNA increased during treatment and recovered to levels comparable to healthy controls. In contrast, mtDNA decreased immediately after the initiation of an AZT\/ddC\u2010containing regimen. We did not find a correlation between mtDNA levels and changes in clinical parameters. There was no significant difference in mtDNA levels between patients with and those without lipoatrophy. Furthermore, there was no obvious difference in mtDNA levels amongst those patients exhibiting signs and symptoms of peripheral neuropathy. In conclusion, the decrease in mtDNA levels in PBMCs amongst HIV\u20101\u2010infected patients and its amelioration by antiretroviral therapy may suggest the influence of direct effects on mitochondria or mtDNA by HIV\u20101 infection. Further investigations are needed to elucidate the mechanisms contributing to decreased mtDNA and the value of mtDNA measurement in the care of HIV\u20101\u2010infected individuals. J. Med. Virol. 70:497\u2013505, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.10423","type":"journal-article","created":{"date-parts":[[2003,6,4]],"date-time":"2003-06-04T10:08:11Z","timestamp":1054721291000},"page":"497-505","source":"Crossref","is-referenced-by-count":77,"title":["Depletion of mitochondrial DNA in HIV\u20101\u2010infected patients and its amelioration by antiretroviral 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