{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:19:15Z","timestamp":1760707155922},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,7,8]],"date-time":"2003-07-08T00:00:00Z","timestamp":1057622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Science Foundation (Hungary)","award":["T022651","T030185"],"award-info":[{"award-number":["T022651","T030185"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2003,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Replication of human cytomegalovirus (HCMV) was investigated in various T\u2010cell lines expressing the <jats:italic>tax<\/jats:italic> gene product of human T\u2010cell leukemia\u2010lymphoma virus type I (HTLV\u2010I). Differential patterns of HCMV replication were found in HTLV\u2010I\u2010carrying cell lines. HCMV gene expression was restricted to the immediate\u2010early genes in MT\u20102 and MT\u20104 cells, whereas full replication cycle of the virus was observed in C8166\u201045 cells. Productive HCMV infection induced a cytopathic effect resulting in the lysis of infected cells. The results of electrophoretic mobility shift assay (EMSA) showed high levels of NF\u2010\u03baB\u2010, CREB\/ATF\u20101\u2010, and SRF\u2010specific DNA binding activity in all Tax\u2010positive cell lines. In contrast, SP1 activity could be detected only in C8166\u201045 cells. Using an inducible system (Jurkat cell line JPX\u20109), a dramatic increase in NF\u2010\u03baB, CREB\/ATF\u20101, SRF, and SP1 binding activity, as well as productive HCMV infection, were observed upon Tax expression. Overexpression of SP1 in MT\u20102 and MT\u20104 cells converted HCMV infection from an abortive to a productive one. These data suggest that the stimulatory effect of Tax protein on HCMV in T cells is accomplished through at least five host\u2010related transcription factor pathways. The results of this study provide possible mechanisms whereby HCMV infections might imply suppression of adult T\u2010cell leukemia. J. Med. Virol. 71:94\u2013104, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.10447","type":"journal-article","created":{"date-parts":[[2003,7,9]],"date-time":"2003-07-09T10:06:37Z","timestamp":1057745197000},"page":"94-104","source":"Crossref","is-referenced-by-count":4,"title":["Differential patterns of human cytomegalovirus gene expression in various T\u2010cell lines carrying human T\u2010cell leukemia\u2010lymphoma virus type I: Role of tax\u2010activated cellular transcription factors"],"prefix":"10.1002","volume":"71","author":[{"given":"Zolt\u00e1n","family":"Beck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attila","family":"B\u00e1csi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangdong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Ebbesen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istv\u00e1n","family":"Andirk\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eszter","family":"Csoma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zsef","family":"K\u00f3nya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etelka","family":"Nagy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferenc 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