{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T00:24:58Z","timestamp":1722990298564},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,7,13]],"date-time":"2001-07-13T00:00:00Z","timestamp":994982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Research Grant OTKA","award":["T-031953"],"award-info":[{"award-number":["T-031953"]}]},{"name":"Faculty of Medicine, University of Debrecen","award":["ETT T-10304, Mec-18"],"award-info":[{"award-number":["ETT T-10304, Mec-18"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sera of blood donors were investigated by a peptide ELISA and indirect immunofluorescence assay to assess the prevalence of HHV\u20108 infection in the Hungarian population. A 14 amino acid long synthetic oligopeptide from the carboxyterminus of orf65\/small virus capsid antigen was used as antigen in the ELISA. ELISA results were confirmed by recombinant orf65 antigen Western blot. Antibodies to the latent nuclear antigen were detected by the immunofluorescence assay. Nine of 12 sera obtained from patients with classical Kaposi sarcoma were reactive by ELISA whereas all were positive by immunofluorescence. Four of 482 (0.83%) healthy blood donors had anti\u2010orf65 peptide antibodies and 17\/1089 (1.56%) had antibodies to the latent nuclear antigen. In a group of children ages 1\u201314 years, antibodies to the latent nuclear antigen (0\/29) were not detected. The prevalence of antibodies to the latent nuclear antigen showed a moderate but significant increase in correlation with senescence. In the Kaposi sarcoma patients, the titre of antibodies to the latent nuclear antigen was significantly higher than in the healthy seropositive donors. The overall HHV\u20108 seroprevalence by the two assays was 2.28% (11\/482) in the Hungarian blood donor group. J. Med. Virol. 64:526\u2013530, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1081","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:49:13Z","timestamp":1030312153000},"page":"526-530","source":"Crossref","is-referenced-by-count":22,"title":["Prevalence and age distribution of human herpesvirus\u20108 specific antibodies in hungarian blood donors"],"prefix":"10.1002","volume":"64","author":[{"given":"Attila","family":"Juh\u00e1sz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9va","family":"Remenyik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zsef","family":"K\u00f3nya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gy\u00f6rgy","family":"Veress","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1gnes","family":"B\u00e9g\u00e1ny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istv\u00e1n","family":"Andirk\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ildik\u00f3","family":"Medgyessy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e1nos","family":"Hunyadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lajos","family":"Gergely","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,7,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"7963","DOI":"10.1128\/jvi.71.10.7963-7968.1997","article-title":"Monocytes 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