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Although the autoradioelectropherograms of each virus were similar, it was possible to differentiate them by their unique protein pattern in the 30,000 to 40,000 molecular weight region of the gels. A single virion glycoprotein (mol. wt. = 38 \u00d7 10<jats:sup>3<\/jats:sup>) and a virion phosphoprotein (mol. wt. = 12 \u00d7 10<jats:sup>3<\/jats:sup>) were associated with each of the virus preparations.<\/jats:p><jats:p>Cross\u2010absorbed monospecific immune sera against variola, monkeypox, and vaccinia virus\u2010infected cells were used in immunodiffusion tests to precipitate radiolabeled, homologous, soluble antigen proteins. The predominant antigen protein associated with each immunospecific precipitate had a molecular weight of approximately 73,000.<\/jats:p>","DOI":"10.1002\/jmv.1890010203","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T07:14:07Z","timestamp":1172819647000},"page":"95-110","source":"Crossref","is-referenced-by-count":27,"title":["The virion and soluble antigen proteins of variola, monkeypox, and vaccinia viruses"],"prefix":"10.1002","volume":"1","author":[{"given":"Joseph J.","family":"Esposito","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Obijeski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James H.","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"373","volume-title":"Viruses of Vertebrates","author":"Andrewes CH","year":"1972"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(75)90010-5"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(65)90030-9"},{"key":"e_1_2_1_5_1","first-page":"215","article-title":"Identification and interrelationships of the variola\/vaccinia subgroup of poxviruses","volume":"19","author":"Baxby D","year":"1975","journal-title":"Progress in Medical Virology"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03599.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.64.5.819"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700590120"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/00327487608069128"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890010107"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-31-3-463"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149755"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/129.3.289"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(67)90072-4"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1975.tb02238.x"},{"key":"e_1_2_1_17_1","first-page":"633","article-title":"Human infection with monkeypox virus: laboratory investigation of six cases in West Africa","volume":"46","author":"Lourie B","year":"1972","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin\u2010phenol reagent","volume":"193","author":"Lowry O","year":"1951","journal-title":"The Journal of Biological Chemistry"},{"key":"e_1_2_1_19_1","first-page":"179","volume-title":"Methods in Virology","author":"Maizel JV","year":"1971"},{"key":"e_1_2_1_20_1","first-page":"613","article-title":"Poxviruses isolated from clinically ill and asymptomatically infected monkeys and a chimpanzee","volume":"46","author":"Marennikova SS","year":"1972","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01245774"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/S0021-9258(19)41829-2","article-title":"Radiolabeling proteins and viruses in vitro by acetylation with radioactive acetic anhydride","volume":"250","author":"Montelaro RC","year":"1975","journal-title":"The Journal of Biological Chemistry"},{"key":"e_1_2_1_23_1","first-page":"405","volume-title":"Comprehensive Virology","author":"Moss B","year":"1974"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(73)90195-2"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(73)81016-5"},{"key":"e_1_2_1_26_1","volume-title":"Comparative Diagnosis of Viral Disease","author":"Nakano JH","year":"1977"},{"key":"e_1_2_1_27_1","first-page":"782","volume-title":"Manual of Clinical Microbiology","author":"Nakano JH","year":"1974"},{"key":"e_1_2_1_28_1","first-page":"374","article-title":"Antigenes solubles des \u201cpox virus.\u201d I. 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