{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T02:19:15Z","timestamp":1744165155867},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":10569,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1977,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present investigation describes the biological and biochemical properties of a persistent SSPE virus infection. Persistently infected cells were derived by cocultivation of infected brain cells and uninfected Vero cells, and cultures were maintained by normal subculturing methods.<\/jats:p><jats:p>No infectious virus was ever released from these cultures, and all attempts to induce infectious virus release were unsuccessful. Biological assays showed that infected cells contained nucleocapsid and salt\u2010dependent hemagglutinin antigens, whereas the normal hemagglutinin appeared not to be present. Electron microscopic examination demonstrated the presence of both intranuclear and cytoplasmic nucleocapsids together with the release of virus particles (defective?) from the cell membrane.<\/jats:p><jats:p>Biochemical analysis demonstrated that approximately 90% of the intracellular genomic RNA was defective or subgenomic although a small quantity of infectious genomes was present.<\/jats:p><jats:p>It is proposed that the large quantities of defective genomes in the infected cells are the major factor in the maintenance of this persistent infection.<\/jats:p>","DOI":"10.1002\/jmv.1890010207","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T07:14:07Z","timestamp":1172819647000},"page":"139-154","source":"Crossref","is-referenced-by-count":21,"title":["Biological and biochemical characterization of a latent subacute sclerosing panencephalitis (SSPE) virus infection in tissue culture"],"prefix":"10.1002","volume":"1","author":[{"given":"Volker","family":"Kratzsch","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William W.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuo","family":"Nagashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Volker","family":"ter Meulen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken1952.25.321"},{"key":"e_1_2_1_3_1","first-page":"241","article-title":"Comparison between productive and latent subacute sclerosing panencephalitis viral infection in vitro","volume":"31","author":"Dubois\u2010Dalcq M","year":"1974","journal-title":"Laboratory Investigation"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1128\/jvi.17.3.1052-1055.1976","article-title":"Induction of measles virus hemagglutinin in a persistently infected, nonvirogenic line of cells","volume":"17","author":"Flanagan TD","year":"1976","journal-title":"Journal of Virology"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02121722"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1128\/jvi.12.4.690-695.1973","article-title":"Activation of a latent measles virus infection in hamster cells","volume":"12","author":"Haspel MV","year":"1973","journal-title":"Journal of Virology"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.18.1_Part_2.008"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-132-34196"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(64)90224-7"},{"key":"e_1_2_1_10_1","first-page":"322","article-title":"Ober die Stellung der Panencephalitis zur Leukoencephaliti sclerosante subaigne. 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