{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T05:21:04Z","timestamp":1699852864801},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:00:00Z","timestamp":1133827200000},"content-version":"vor","delay-in-days":9836,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1979,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Physicochemical studies of hepatitis B e antigen (HBeAg)<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#note1\" \/><jats:fn><jats:p>Both e<jats:sub>1<\/jats:sub> and e<jats:sub>2<\/jats:sub> antigens were included in the terminology of HBeAg or e antigen in the article for easy understanding. The e<jats:sub>2<\/jats:sub> antigen corresponds to Magnius and Espmark's original e antigen and this should be called HBe antigen in the future (authors' opinion).<\/jats:p><\/jats:fn>\n revealed a clear cut difference between e<jats:sub>1<\/jats:sub> and e<jats:sub>2<\/jats:sub> antigen. The e<jats:sub>1<\/jats:sub> antigen was found to have a MW of Ca 150,000 and a pI of 6.4\u20137.2, whereas both the MW and pI of the e<jats:sub>2<\/jats:sub> antigen were heterogeneous depending upon the source of serum.<\/jats:p><jats:p>Sera obtained from asymptomatic carriers were characterized by low titers of HBs antigen, HBc antigen and DNA polymerase and contained e<jats:sub>2<\/jats:sub> antigen of larger molecular weight (200,000\u2013300,000) with a narrow distribution range and a pI of 4.8 to 5.2 (type 1). On the other hand, the sera from patients in a hemodialysis unit who were HBs antigen carriers and had high titers of HBs antigen, HBc antigen and DNA polymerase contained e<jats:sub>2<\/jats:sub> antigen of heterogeneous distribution in MW (from 300,000 to 70,000) and pI (type 2 and 3). The e<jats:sub>2<\/jats:sub> antigen obtained from the higher MW type 3 serum had lower isoelectric points (pI 4.5 to 5.2) as was the case with e<jats:sub>2<\/jats:sub> antigen obtained from asymptomatic carriers whereas a relatively wide range of isoelectric points (pI 5.1 to 8.2) was found with the lower molecular weight e<jats:sub>2<\/jats:sub> antigen.<\/jats:p>","DOI":"10.1002\/jmv.1890040105","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T08:44:57Z","timestamp":1172825097000},"page":"33-42","source":"Crossref","is-referenced-by-count":4,"title":["Physicochemical heterogeneity of hepatitis B e antigen detected in asymptomatic carriers and carriers in a hemodialysis unit"],"prefix":"10.1002","volume":"4","author":[{"given":"Ei","family":"Yamada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Ohori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nakao","family":"Ishida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1977.tb01573.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.4049\/jimmunol.104.4.1031","article-title":"Rapid detection of Australia antigen by counter\u2010immunoelectrophoresis","volume":"104","author":"Gocke D","year":"1970","journal-title":"The Journal of 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