{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T22:40:41Z","timestamp":1699828841386},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:00:00Z","timestamp":1133827200000},"content-version":"vor","delay-in-days":9836,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1979,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A cytopathic measles virus was isolated from a brain biopsy of a subacute sclerosing panencephalitis (SSPE) patient. The agent could be transferred to Vero cells by cocultivation, but the infectivity always remained cell\u2010associated \u2014 ie, a defective virus infection. The cell\u2010associated nature of the virus was retained through 25 passages in Vero cells. Intracerebral inoculation of hamsters (2\u20146 days old) with the cocultured Vero cells gave rise to 100% mortality in 5\u20147 days. The virus retained its cell\u2010associated nature after passage in hamsters. Electron microscopy of the brain and Vero cocultures showed the presence of virus\u2010like ribonucleoparticles mainly in the nucleus. The presence of viral antigens in the nucleus, cytoplasm, and on the plasma membranes was confirmed by immunofluorescence. Using a combination of immunological and biochemical technqiues, it was shown that all the viral proteins were synthesized with the exception of the haemagglutinin.<\/jats:p><jats:p>Inclusion of the fusion inhibitor SV4814 (CBZ\u2010D phenylalanine\u2010L\u2010phenylalanine\u2010L\u2010arginine\u2010NO<jats:sub>2<\/jats:sub>) in the culture medium led to the elimination of the SSPE infection.<\/jats:p>","DOI":"10.1002\/jmv.1890040205","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T09:08:35Z","timestamp":1172826515000},"page":"103-114","source":"Crossref","is-referenced-by-count":7,"title":["Isolation and characterisation of a defective measles virus from a subacute sclerosing panencephalitis patient"],"prefix":"10.1002","volume":"4","author":[{"given":"T. F.","family":"Wild","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Giraudon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Huppert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-129-33377"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03599.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-21-2-407"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.163.3872.1193"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken1952.25.321"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(78)90025-9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/272460a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/221974a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/222888a0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01250213"},{"key":"e_1_2_1_12_1","first-page":"1183","article-title":"Subacute sclerosing panencephalitis: Isolation of a measles\u2010like virus in tissue culture of brain biopsy","volume":"103","author":"Kettyls GD","year":"1970","journal-title":"Canadian Medical Association Journal"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000148928"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-111-27930"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(71)90374-6"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/272458a0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149471"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-39-1-113"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149045"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio240122a0"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890040205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890040205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T22:16:36Z","timestamp":1699827396000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890040205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,1]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1979,1]]}},"alternative-id":["10.1002\/jmv.1890040205"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890040205","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1979,1]]}}}