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The purity of this preparation was 90\u2010 to 100\u2010fold higher than that of a previously used partially purified vaccine. A mean protein content of 3\u20135 m\u0308g\/ml in the final vaccine represents 20 mouse protective doses<jats:sub>50<\/jats:sub> in a mouse challenge potency test. This high degree of purity resulted in a strong reduction of side reactions observed with the less purified vaccine.<\/jats:p>","DOI":"10.1002\/jmv.1890060304","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T10:13:03Z","timestamp":1172830383000},"page":"213-221","source":"Crossref","is-referenced-by-count":68,"title":["Preparation of a highly purified vaccine against tick\u2010borne encephalitis by continuous flow zonal ultracentrifugation"],"prefix":"10.1002","volume":"6","author":[{"given":"Franz 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