{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T16:43:42Z","timestamp":1782319422969,"version":"3.54.5"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T00:00:00Z","timestamp":1133827200000},"content-version":"vor","delay-in-days":9105,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1981,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A sensitive enzyme immunoassay is described for demonstrating specific antibodies of the IgM class to tick\u2010borne encephalitis virus (anti\u2010TBEV IgM). Anti\u2010m\u0308\u2010coated, flat\u2010bottomed microtiter plates are incubated with diluted patients'serum (2 hr at 37\u00b0C), then with purified TBEV, and later with pero\u2010xidase\u2010coupled anti\u2010TBEV immunoglobulin for a further 2 hr. After washing the plates, orthophenylenediamine is added and the optical density is measured at 510 nm. At an early stage after onset of illness anti\u2010TBEV IgM could be demonstrated up to serum dilutions of 10<jats:sup>\u22124<\/jats:sup>. The specificity of the test system was shown by total inhibition of the positive reaction by preincubation of patients'sera with anti\u2010m\u0308. Tick\u2010borne encephalitis (TBE) could be diagnosed serologically in 54 patients by the described method, and anti\u2010TBEV IgM could be demonstrated in sera drawn up to 9 months after onset of illness. A peak in the number of infections occurs in the age group of 31\u201340 years, and most infections occur from June to October with a small peak in July.<\/jats:p>","DOI":"10.1002\/jmv.1890070105","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T10:11:08Z","timestamp":1172830268000},"page":"41-50","source":"Crossref","is-referenced-by-count":38,"title":["Serological diagnosis of acute tick\u2010borne encephalitis by demonstration of antibodies of the IgM 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by treatment of serum with 2\u2010mercaptoethanol","volume":"218","author":"Kunz Ch","year":"1971","journal-title":"Zentralblatt f\u00fcr Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Erste Abteilung\/Originale"},{"key":"e_1_2_1_11_2","first-page":"2195","article-title":"Klinisch\u2010epidemiologische Bedeutung der Arboviren in Zentraleuropa","volume":"71","author":"Kunz Ch","year":"1976","journal-title":"Medizinische Klinik"},{"key":"e_1_2_1_12_2","first-page":"281","article-title":"Untersuchungen in einem Naturherd der Fr\u00fchsommer\u2010Meningoenzephalitis (FSME) in Nieder\u00f6sterreich","volume":"190","author":"Radda A","year":"1963","journal-title":"Zentralblatt f\u00fcr Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Erste Abteilung\/Originale"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890050107"},{"key":"e_1_2_1_14_2","unstructured":"RoggendorfM DeinhardtF Fr\u00f6snerGG ScheidR BayerlB ZachovalR: (1981): IgM antibodies to hepatitis B core antigen: Evaluation of test and diagnostic use in hepatitis B.Journal of Clinical Microbiology in press."},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-50-1-59"},{"key":"e_1_2_1_16_2","first-page":"277","article-title":"Zentralcurop\u00e4ische Enzephalitis (\u201cZeckenenzephalitis\u201d) in der Schweiz","volume":"99","author":"Spiess H","year":"1969","journal-title":"Schweizer medizinische Wochenschrift"},{"key":"e_1_2_1_17_2","first-page":"215","volume-title":"Immunofluorescence and Related Staining Techniques","author":"Wilson MB","year":"1978"}],"container-title":["Journal of Medical 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