{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:37:00Z","timestamp":1760204220878},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":7494,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1985,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this work was to elucidate the pathogenesis of attenuated Junin virus (JV) strains in the guinea pig model. Three groups of guinea pigs were infected by the IM route with 10<jats:sup>3<\/jats:sup> PFU of the XJC13 and XJO\u2010attenuated strains or with the XJ pathogenic strain of JV, respectively. Viremia was studied at 3, 5, 7, 9, 12, and 14 days postinfection (pi) (a) in serum samples of all animals and in washed cells from XJC13\u2010infected guinea pigs by conventional techniques and (b) in whole blood samples from XJC13 and XJO animals by coculture with Vero cells. Virus spread was studied at 14 days pi in brain, spleen, lymph nodes, and bone marrow by parallel suckling mouse inoculation or organ homogenates and coculture of cell suspensions with Vero cells. By coculture techniques of whole blood, an otherwise undetectable viremia was demonstrated for both attenuated strains throughout the observation period. In contrast, XT viremia was easily detected by direct techniques, as has already been shown. Attenuated virus was also shown to reach brain and bone marrow when coculture methods were employed. But titers were always markedly lower than those of the pathogenic strain. The sustained viremia demonstrated in guinea pigs infected with either attenuated strain explains the mode of viral dissemination and accounts for viral rescue and antigen detection from some organs.<\/jats:p><jats:p>These results suggest that attenuated strains do not differ greatly in their invasive capacity in guinea pigs, but later on viral replication is impaired. Therefore, these findings reveal potential risks and should be noted when developing human vaccines.<\/jats:p>","DOI":"10.1002\/jmv.1890150212","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T12:00:16Z","timestamp":1172836816000},"page":"197-202","source":"Crossref","is-referenced-by-count":5,"title":["Pathogenesis of attenuated Junin virus in the guinea pig model"],"prefix":"10.1002","volume":"15","author":[{"given":"Luc\u00eda B.","family":"de Guerrero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha C.","family":"Boxaca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ester","family":"Malumbres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda de las Mercedes","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"issue":"2","key":"e_1_2_1_2_1","first-page":"745","article-title":"Aislamiento de virus Jun\u00edn por cocultivo de leucocitos perif\u00e9ricos de enfermos de fiebre hernorr\u00e1gica argentina","volume":"42","author":"Ambrosio AM","year":"1983","journal-title":"Medicina (Bs. Aires)"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149219"},{"key":"e_1_2_1_4_1","first-page":"157","article-title":"Selectividad tisular e indicadores de virulencia de tres cepas del virus Jun\u00edn","volume":"41","author":"Avila MM","year":"1981","journal-title":"Medicina (Bs. Aires)"},{"key":"e_1_2_1_5_1","first-page":"255","article-title":"Rescue of Junin virus from adult mice by cocultivation procedures","volume":"2","author":"Berr\u00eda MI","year":"1983","journal-title":"Comunicaciones biol6gicas"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149517"},{"key":"e_1_2_1_7_1","first-page":"521","article-title":"Algunos aspectos de la infecci\u00f3n experimental del cobayo con una variante atenuada dcl virus Jun\u00edn","volume":"40","author":"Boxaca MC","year":"1980","journal-title":"Medicina (Bs. Aires)"},{"key":"e_1_2_1_8_1","first-page":"230","article-title":"Viremia en enfermos de fiebre hemorr\u00e1gica argentina","volume":"79","author":"Boxaca MC","year":"1965","journal-title":"Revista de la Asociaci\u00f3n M\u00e9dica Argentina"},{"key":"e_1_2_1_9_1","first-page":"170","article-title":"Fiebre hemorr\u00e1gica experimental en el cobayo (virus Junin)","volume":"37","author":"Boxaca MC","year":"1961","journal-title":"Revista de la Sociedad Argentina de Biolog\u00eda"},{"key":"e_1_2_1_10_1","first-page":"8","article-title":"Estudio de la infecci\u00f3n experimental del rat\u00f3n por virus Jun\u00edn: enfermedad tipo","volume":"7","author":"Giovanniello OA","year":"1975","journal-title":"Revista de la Asociaci\u00f3n Argentina de Microbiolog\u00eda"},{"key":"e_1_2_1_11_1","first-page":"250","article-title":"Lymphatic tissue in Argentine hemorrhagic fever: Pathologic features","volume":"104","author":"Gonz\u00e1lez PH","year":"1980","journal-title":"Archives of Pathology and Laboratory Medicine"},{"key":"e_1_2_1_12_1","first-page":"267","article-title":"Estudio preliminar de una variante atenuada de virus Jun\u00edn derivada de la cepa prototipo XJ","volume":"40","author":"Guerrero de LB","year":"1980","journal-title":"Medicina (Bs Aires)"},{"key":"e_1_2_1_13_1","first-page":"271","article-title":"Infecci\u00f3n experimental del cobayo con virus Jun\u00edn, Cuadro cl\u00ednico, diseminaci\u00f3n y eliminaci\u00f3n de virus","volume":"371","author":"Guerrero de LB","year":"1977","journal-title":"Medicina (Bs. Aires)"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1128\/iai.41.3.1279-1283.1983","article-title":"In vivo replication of pathogenic and attenuated strains of Junin virus in different cell population of lymphatic tissue","volume":"41","author":"Laguens M","year":"1983","journal-title":"Infection and Immunity"},{"key":"e_1_2_1_15_1","first-page":"231","article-title":"Inmunizaci\u00f3n contra la fiebre hemorr\u00e1gica argentina con una cepa atenuada de virus Jun\u00edn. Anilisis de 636 vacunados","volume":"61","author":"Rugiero HR","year":"1974","journal-title":"Prensa M\u00e9dica Argentina"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.4049\/jimmunol.48.2.111","article-title":"Recovery of the virus of LCM from the erythrocyte of infected animals","volume":"48","author":"Schwartzman G","year":"1944","journal-title":"Journal of Immunology"},{"key":"e_1_2_1_17_1","unstructured":"WeissenbacherMC AvilaMM CalelloMA SamoilovichSR CotoCE Guerrero deLB FrigerioMJ(1982):Vacunas experimentales contra la fiebre hemorr\u00e1gica argentina en primates no humanos: Callithrix jacchus. Resumen N: G\u2010193 III Congreso Argentino de Microbiolog\u00eda Bs.As."},{"key":"e_1_2_1_18_1","first-page":"21","article-title":"Infecci\u00f3n de primates del nuevo mundo con virus Jun\u00edn. II Callithrix jacchus","volume":"40","author":"Weissenbacher MC","year":"1980","journal-title":"Medicina (Bs. 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