{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:03:16Z","timestamp":1762160596172},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,11]],"date-time":"2005-12-11T00:00:00Z","timestamp":1134259200000},"content-version":"vor","delay-in-days":7468,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1985,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have examined the distribution of human papillomavirus (HPV) DNA in paraffin sections of human warts by in situ hybridization with biotin\u2010labeled DNA probes. Recombinant plasmid DNAs (HPV\u20101, \u22126, \u221211, \u221216) were labeled by nick translation with biotinylated deoxyuridine triphosphate. Paraffin sections were hybridized with the probes for 18 h in stringent or nonstringent conditions, and DNA\u2010DNA hybrids were detected by immunocytochemistry. Paraffin sections of warts were also examined for the presence of HPV capsid antigen with the avidinbiotin peroxidase complex method for immunocytochemistry.<\/jats:p><jats:p>HPV DNA was detected and localized in paraffin sections from a plantar wart, a laryngeal papilloma, and seven anogenital condylomas. The specific HPV type present in each lesion was determined by hybridization under stringent conditions with the homologous DNA probe. The papillomas were found to contain many more cells with replicating virus DNA, as demonstrated by in situ hybridization, than was apparent from the number of cells containing detectable virus antigen. In situ hybridization with biotin\u2010labeled probes is an effective technique for the identification of HPV infection in routinely collected and processed tissue specimens.<\/jats:p>","DOI":"10.1002\/jmv.1890160307","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T12:09:27Z","timestamp":1172837367000},"page":"265-273","source":"Crossref","is-referenced-by-count":118,"title":["Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes"],"prefix":"10.1002","volume":"16","author":[{"given":"Anna Marie","family":"Beckmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Myerson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet R.","family":"Daling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy B.","family":"Kiviat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia M.","family":"Fenoglio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James K.","family":"McDougall","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,11]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"126","volume-title":"Sexually Transmitted Diseases","author":"Aral SO","year":"1984"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01944.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890120304"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(83)90460-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(82)90341-5"},{"key":"e_1_2_1_7_1","first-page":"545","article-title":"Workshop on papillomaviruses and cancer","volume":"39","author":"Coggin JR","year":"1979","journal-title":"Cancer Research"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198404053101403"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1128\/jvi.40.3.932-935.1981","article-title":"Molecular cloning of viral DNA from human genital warts","volume":"4126","author":"deVilliers E\u2010M","year":"1981","journal-title":"Journal of Virology"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3812"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910290205"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1128\/jvi.44.1.393-400.1982","article-title":"Molecular cloning and characterization of human papillomavirus DNA derived from a laryngeal papilloma","volume":"44","author":"Gissmann L","year":"1982","journal-title":"Journal of Virology"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.2.560"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","unstructured":"GuptaJ GendelmanHE NaghashfarZ GuptaP RosensheinN SawadaE WoodruffJD ShahKV(1984): Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situ hybridization with radioactive probes: A preliminary communication. 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