{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:18:36Z","timestamp":1777475916277,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,11]],"date-time":"2005-12-11T00:00:00Z","timestamp":1134259200000},"content-version":"vor","delay-in-days":7437,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1985,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An indirect ELISA and an inhibition ELISA were developed for the detection of cytomegalovirus (CMV)\u2010specific immunoglobulin G (IgG) and CMV\u2010specific total immunoglobulin, respectively. Both assays were more specific than the complement fixation (CF) test, and titres of positive sera were 660 times higher by IgG ELISA and 6 times higher by inhibition ELISA than titres by the CF test. Titres by IgG ELISA were reliably determined using the absorbance obtained at a single serum dilution of 1\/1,000 in conjunction with a standard graph. Both ELISAs compared favourably with each other in sensitivity and specificity in determining CMV immune status. The inhibition ELISA, in particular, provides a simple and reliable method of screening sera, which requires no control antigen or predilution of sera. It should prove useful for large\u2010scale screening procedures, such as blood donor testing.<\/jats:p>","DOI":"10.1002\/jmv.1890160411","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T12:08:06Z","timestamp":1172837286000},"page":"375-384","source":"Crossref","is-referenced-by-count":3,"title":["Quantitative and qualitative detection of cytomegalovirus\u2010specific antibodies using two types of enzyme\u2010linked immunosorbent assay"],"prefix":"10.1002","volume":"16","author":[{"given":"Kevin A.","family":"Kinane","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene B.","family":"Hillary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/pgmj.54.628.112"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1128\/am.21.1.84-89.1971","article-title":"Indirect haemagglutination test for detecting antibodies to cytomegalovirus","volume":"21","author":"Bernstein MT","year":"1971","journal-title":"Applied Microbiology"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1975.131"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1128\/jcm.4.2.151-156.1976","article-title":"Comparative activity of immunofluorescent antibody in cytomegalovirus infection","volume":"4","author":"Betts RF","year":"1976","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.32.2.122"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890050303"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.35.12.1345"},{"key":"e_1_2_1_9_1","first-page":"96","article-title":"Technique of complement fixation test applicable to the diagnosis of virus diseases","volume":"21","author":"Bradstreet CMP","year":"1962","journal-title":"Monthly Bulletin of the Ministry of Health and Public Health Laboratory Service"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.4.5684.660"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/123.5.555"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(80)90009-9"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1128\/jcm.6.6.647-649.1977","article-title":"Improved glycine\u2010extracted complement\u2010fixing antigen for human cytomegalovirus","volume":"6","author":"Kettering JD","year":"1977","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1128\/jcm.18.1.33-39.1983","article-title":"Normalised enzyme\u2010linked immunosorbent assay for determining immunoglobulin G antibodies to cytomegalovirus","volume":"18","author":"Kiefer DJ","year":"1983","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-79-2-267"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"2006","DOI":"10.4049\/jimmunol.117.5_Part_2.2006","article-title":"Cytomegalovirus specific IgM and IgG response in humans studied by radioimmunoassay","volume":"117","author":"Knez V","year":"1976","journal-title":"Journal of Immunology"},{"key":"e_1_2_1_17_1","first-page":"605","article-title":"A collaborate study of cytomegalovirus antibodies in mothers and young children in 19 countries","volume":"59","author":"Krech U","year":"1981","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)90345-3"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(66)92929-1"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/22.12.1084"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/136.6.754"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-198007000-00004"},{"key":"e_1_2_1_23_1","first-page":"187","article-title":"The respective advantages of complement fixation and ELISA for routine diagnosis of cytomegalovirus infection","volume":"35","author":"Pozzetto B","year":"1981","journal-title":"Biomedicine"},{"key":"e_1_2_1_24_1","first-page":"1","article-title":"Enzyme linked immunosorbent assay (ELISA) for determination of IgG antibodies to human cytomegalovirus","volume":"88","author":"Sarov I","year":"1980","journal-title":"Acta Pathologica et Microbiologica Scandinavica"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(77)90410-7"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(81)90017-3"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.34.6.665"},{"key":"e_1_2_1_28_1","first-page":"243","article-title":"Enzyme\u2010immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination","volume":"57","author":"Voller A","year":"1976","journal-title":"British Journal of Experimental Pathology"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197107222850406"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(81)80662-2"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890160411","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890160411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T19:01:01Z","timestamp":1697828461000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890160411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,8]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1985,8]]}},"alternative-id":["10.1002\/jmv.1890160411"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890160411","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,8]]}}}