{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:22:48Z","timestamp":1761128568394},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":6915,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The availability of cloned varicella\u2010zoster virus (VZV) DNA probes allows rapid detection of viral\u2010specific DNA by \u201cdot\u2010blot\u201d hybridization in lymphocytes or in lesion aspirates. Thirty\u2010six blood specimens were obtained from 25 patients with suspected varicella or zoster. VZV\u2010specific DNA was demonstrated in 15 lymphocyte preparations of nine patients with varicella and in one with disseminated zoster out of five patients with zoster. VZV\u2010specific DNA was detected prior to rise in antibodies, indicating early viremia in these patients. Virus isolation from lesions and serological tests confirmed VZV infections. VZV\u2010specific DNA was detected in lymphocytes of three patients out of six with active herpetic lesions, whereas it was not detected in lymphocyte specimens from two patients with undiagnosed rash or four with lymphoproliferative diseases, who did not present varicella or zoster, or from 18 healthy controls. No signal was obtained in herpes simplex virus (HSV)\u2010infected and \u2010uninfected cell lines. The hybridization assay proved that specific and viral or cellular DNAs other than VZV did not crosshybridize with the probe. The sensitivity limit of detection was 4\u201315 pg of homologous DNA, and the assay was accomplished within 72\u201396 hr. These results point to the possible rapid diagnosis of VZV infection in patients suspected of varicella or generalized zoster. In addition, simultaneous infection with both VZV and HSV seems to occur in some patients.<\/jats:p>","DOI":"10.1002\/jmv.1890210108","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T13:09:15Z","timestamp":1172840955000},"page":"57-66","source":"Crossref","is-referenced-by-count":30,"title":["Detection of varicella\u2010zoster virus in lymphocytes by DNA hybridization"],"prefix":"10.1002","volume":"21","author":[{"given":"A.","family":"Vonsover","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Leventon\u2010Kriss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Langer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Smetana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Gotlieb\u2010Stematsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Zaizov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Potaznick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. 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