{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T21:39:42Z","timestamp":1744148382931},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":6856,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1987,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To assess the usefulness of the South American primate <jats:italic>Cebus apella<\/jats:italic> as a model for neurovirulence of Jun\u00edn virus, eight monkeys were inoculated with 10<jats:sup>5<\/jats:sup> LD<jats:sub>50<\/jats:sub> of the attenuated XJ\u2010Clone 3 Jun\u00edn virus strain by the intrathalamic route. After the second week, weight loss and polyadenopathies were observed in most animals, one\u2010half of which had a transient leukothrombocytopenia.<\/jats:p><jats:p>Moderate clinical central nervous system (CNS) involvement was present in four of eight monkeys, while the rest had only mild neurologic signs. All recovered except one, which developed a deep coma and was killed in a pre\u2010mortem stage at 18 days post\u2010infection (pi).<\/jats:p><jats:p>Jun\u00edn virus was isolated from the throat from five, from the blood from three, and from the brain from two monkeys. In the most severely ill animal, virus titers higher than viremia were detected in both inoculated and contralateral brain hemispheres, as well as in lung, lymph node, and small intestine.<\/jats:p><jats:p>Jun\u00edn antigens and \u201cin vivo\u201d bound immunoglobulins were detected by immunofluorescence (IF) in the brain of four animals at 18, 21, 40, and 155 days pi. Moderate lymphocytic parenchymal and meningeal infiltration were observed in the brain of four animals, and gliosis was also present in the most affected monkey. Although the clinical response to infection was not uniform, all infected monkeys developed high IF antibodies.<\/jats:p><jats:p><jats:italic>Cebus apella<\/jats:italic> cannot be used as a highly sensitive model for Argentine hemorrhagic fever (AHF). However, the results obtained show that the XJ\u2010Clone 3 strain can replicate in the primate CNS and to induce lesions and immunoglobulin deposition. In addition, viral persistence is suggested by the late detection of viral antigens in brain at 40 and 155 days pi.<\/jats:p>","DOI":"10.1002\/jmv.1890210309","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T13:22:18Z","timestamp":1172841738000},"page":"257-268","source":"Crossref","is-referenced-by-count":11,"title":["Intracerebral infection of cebus apella with the XJ\u2010clone 3 strain of Jun\u00edn virus"],"prefix":"10.1002","volume":"21","author":[{"given":"Guadalupe","family":"Carballal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 R.","family":"Oubi\u00f1a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Videla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felisa C.","family":"Molinas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Nagle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda T.","family":"De La Vega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Elsner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890150112"},{"key":"e_1_2_1_3_1","first-page":"284","article-title":"Efecto de la inoculaci\u00f3n intracerebral del virus Jun\u00edn en el cobayo","volume":"42","author":"Boxaca MC","year":"1982","journal-title":"Medicina (Buenos Aires)"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1950.3.6.365"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/143.1.7"},{"key":"e_1_2_1_6_1","first-page":"639","article-title":"Infecci\u00f3n experimental de un primate sudamericano, el Cebus sp. con la cepa XJ de virus Jun\u00edn","volume":"43","author":"Carballal G","year":"1983","journal-title":"Medicina (Buenos Aires)"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149180"},{"key":"e_1_2_1_8_1","first-page":"503","article-title":"Presencia de ant\u00edpeno de virus Jun\u00edn e inmunoglobulinas depositadas \u201cin vivo\u201d en el sistema nervioso central de cuatro casos fatales de fiebre hemorr\u00e1gica argentina","volume":"37","author":"Cossio PM","year":"1977","journal-title":"Medicina (Buenos Aires)"},{"key":"e_1_2_1_9_1","first-page":"1","article-title":"Inmunizaci\u00f3n contra la fiebre hemorr\u00e1gica argentina con una cepa atenuada del virus Jun\u00edn. 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Inmunizaci\u00f3n de cobayos","volume":"29","author":"de Guerrero LB","year":"1969","journal-title":"Medicina (Buenos Aires)"},{"key":"e_1_2_1_10_1","first-page":"481","article-title":"Method for platelet detection","volume":"4","author":"Feissly R","year":"1949","journal-title":"Revue Hematologie"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(79)92335-3"},{"key":"e_1_2_1_12_1","first-page":"144","article-title":"Infection of Cchs monkey with Jun\u00edn virus","volume":"45","author":"McKee KT","year":"1985","journal-title":"Medicina (Buenos Aires)"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890120408"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890150208"},{"key":"e_1_2_1_15_1","first-page":"376","volume-title":"Hemorrhagic Disease","author":"Quick AJ","year":"1957"},{"key":"e_1_2_1_16_1","first-page":"81","article-title":"Inmunizaci\u00f3n contra la fiebre hemorr\u00e1gica argentina con una cepa atenuada de virus Jun\u00edn. 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