{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T06:29:30Z","timestamp":1715840970571},"reference-count":10,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":6825,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Since outbreaks of severe acute hemorrhagic conjunctivitis occur worldwide [Hierholzer and Hatch], 19851 and the majority of the epidemics are caused by enterovirus 70 (EV\u201070), we developed an EV\u201070 IgM ELISA to simplify the diagnosis of these outbreaks. The test is based on the capture antibody technique and the use of monoclonal antibodies to EV\u201070. We detected EV\u201070 IgM antibodies in 55% of 76 convalescent\u2010phase sera from an outbreak of acute hemorrhagic conjunctivitis in a Brazilian community. Among the 71 acute\u2010 and convalescentphase serum pairs from this outbreak, 49 (69%) demonstrated a 4\u2010fold or greater rise in neutralizing antibody titer. The titer of IgM antibody began to drop by the fifth week after onset of illness. EV\u201070 IgM antibodies were not detected in 53 serum pairs with a 4\u2010fold or greater rise in antibodies to other picornaviruses. The EV\u201070 ELISA proved to simple and relatively rapid to perform, appeared to be specific, and should be sensitive enough to diagnose outbreaks of EV\u201070 when multiple serum specimens can be tested.<\/jats:p>","DOI":"10.1002\/jmv.1890210404","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T13:19:10Z","timestamp":1172841550000},"page":"321-327","source":"Crossref","is-referenced-by-count":7,"title":["Diagnosis of enterovirus 70 infection by demonstration of IgM antibodies"],"prefix":"10.1002","volume":"21","author":[{"given":"Herta","family":"Wulff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry J.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Pallansch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato Piza","family":"De Souza Carvalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1128\/jcm.20.3.405-408.1984","article-title":"Detection of enterovirus 70 with monoclonal antibodies","volume":"20","author":"Anderson LJ","year":"1984","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)91843-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1128\/jcm.21.5.830-834.1985","article-title":"Comparison of serodiagnosis of group B Coxsackievirus infections by an immunoglobulin M capture enzyme immunoassay versus microneutralization","volume":"21","author":"Chan D","year":"1985","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1128\/jcm.19.6.826-830.1984","article-title":"Quantitation of enterovirus 70 antibody by microneutralization test and comparison with standard neutralization, hemagglutination inhibition, and complement fixation tests with different virus strains","volume":"19","author":"Hierholzer JC","year":"1984","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_6_1","volume-title":"Viral Diseases of the Eye","author":"Hierholzer JC","year":"1985"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)92353-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/135.5.706"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149369"},{"key":"e_1_2_1_10_1","first-page":"485","article-title":"Immunofluorescence test for the epidemiological monitoring of acute hemorrhagic conjunctivitis cases","volume":"61","author":"Pal SR","year":"1983","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1128\/jcm.20.5.972-976.1984","article-title":"Comparison of nonspecific reactivity in indirect and reverse immunoassays for measles and mumps immunoglobulin M antibodies","volume":"20","author":"Tuokko H","year":"1984","journal-title":"Journal of Clinical Microbiology"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890210404","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890210404","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T13:05:11Z","timestamp":1697893511000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890210404"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4]]},"references-count":10,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4]]}},"alternative-id":["10.1002\/jmv.1890210404"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890210404","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4]]}}}