{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:03:16Z","timestamp":1762160596912},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":6825,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1987,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Specific varieties of human papillomavirus (HPV) infecting the anogenital region were detected in clinical samples by use of a filter hybridization technique suitable for rapid screening of cervical and anal scrapes. In this way possibly benign types (HPV6 and HPV11) could be differentiated from types thought to be capable of malignant transformation (HPV16 and HPV18). Cervical or anal canal cells were applied directly to nylon filters and fixed by u.v. irradiation before hybridization with mixed viral DNA probes under both low\u2010 and high\u2010stringency conditions. In addition, probe for the human Alu\u2010repeated DNA sequence was used to assess the relative amount of total nucleic acids in each sample applied to the filter. HPV DNA was detected in 3 of 19 cervical scrapes from patients with no past or present history of wart virus infection or cervical dysplasia. Within a positive study group totalling 71 patients, HPV (6\/11 or 16\/18) was detected in cervical scrapes from 24% of 41 patients who did not have visible genital dysplasia, 30% of 27 patients with visible genital dysplasia or cervical intraepithelial neoplasia (CIN) I, and in 1 of 3 patients with past CIN IUIII. In addition, HPV6\/11 or 16\/18 DNA was detected in anal scrapes from 3 of 6 male patients and in 85% of genital biopsies. A notably high proportion (4\/6) of vaginal condylomata were positive with both the HPV6\/11 and the HPV16\/18 mixed viral DNA probes. Of the biopsies prepared for histopathology and positive for HPV DNA, the HPV group\u2010specific antigen could be detected in only 60%.<\/jats:p>","DOI":"10.1002\/jmv.1890210410","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T13:19:10Z","timestamp":1172841550000},"page":"381-393","source":"Crossref","is-referenced-by-count":21,"title":["Detection of specific types of human papillomavirus in cervical scrapes, anal scrapes, and anogenital biopsies by DNA hybridization"],"prefix":"10.1002","volume":"21","author":[{"given":"Beric R.","family":"Henderson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian J.","family":"Morris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol H.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara R.","family":"Rose","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne E.","family":"Cossart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890160307"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(85)90142-5"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01944.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)91215-0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-64-7-1509"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)91724-6"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19820201)49:3<468::AID-CNCR2820490313>3.0.CO;2-7"},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1128\/jvi.40.3.932-935.1981","article-title":"Molecular cloning of viral DNA from human genital warts","volume":"40","author":"de Villiers EM","year":"1981","journal-title":"Journal of Virology"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.287.6391.510"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3812"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198509263131304"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"26s","DOI":"10.1038\/jid.1984.16","article-title":"Presence of human papilloma virus in genital tumours","volume":"83","author":"Gissmann L","year":"1984","journal-title":"Journal of Investigative Dermatology"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.44.1.393-400.1982"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.2.560"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12522852"},{"key":"e_1_2_1_17_1","first-page":"495","article-title":"Immunologic relatedness of papilloma viruses from different species","volume":"64","author":"Jenson AB","year":"1980","journal-title":"Journal of the National Cancer Institute"},{"key":"e_1_2_1_18_1","first-page":"1091","article-title":"Perspectives on the epidemiology of cervical cancer with special reference to the herpes virus hypothesis","volume":"34","author":"Kessler II","year":"1974","journal-title":"Cancer Research"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1128\/jvi.32.1.199-207.1979","article-title":"Conserved polynucleotide sequences among the genomes of papillomaviruses","volume":"32","author":"Law M\u2010F","year":"1979","journal-title":"Journal of Virology"},{"key":"e_1_2_1_20_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"e_1_2_1_21_1","first-page":"505","article-title":"Condylomatous lesions of the cervix and vagina. I. Cytologic patterns","volume":"20","author":"Meisels A","year":"1976","journal-title":"Acta Cytologia"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(77)90052-3"},{"key":"e_1_2_1_23_1","first-page":"1353","article-title":"A comparison review of key epidemiologic studies in cervical cancer related to current searches for transmissable agents","volume":"33","author":"Rotkin ID","year":"1973","journal-title":"Cancer Research"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6281889"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910350405"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_1_27_1","first-page":"767","article-title":"Identification of human papilloma virus in cervical smears by deoxyribonucleic acid in situ hybridization","volume":"64","author":"Wagner D","year":"1984","journal-title":"Obstetrics and Gynecology"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)91963-4"},{"key":"e_1_2_1_29_1","first-page":"727","article-title":"Immunological identification of papilloma virus antigen in paraffin\u2010processed condyloma tissue from the female genital tract","volume":"56","author":"Woodruff JD","year":"1980","journal-title":"Obstetrics and Gynecology"},{"key":"e_1_2_1_30_1","first-page":"170","article-title":"Viruses in the aetiology of human genital cancer","volume":"30","author":"zur Hausen H","year":"1984","journal-title":"Progress in Medical Virology"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890210410","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890210410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T13:05:01Z","timestamp":1697893501000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890210410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,4]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1987,4]]}},"alternative-id":["10.1002\/jmv.1890210410"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890210410","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,4]]}}}