{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:36:05Z","timestamp":1775151365523,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":6703,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1987,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An enzyme immunoassay (EIA) has been developed which detects antigen(s) (Ag) of the human immunodeficiency virus (HIV) in the serum of patients with the acquired immunodeficiency syndrome (AIDS), AIDS\u2010related complex (ARC), and patients at high risk for HIV infection. The test has a sensitivity of approximately 50 pg\/ml of HIV protein. The specificity of the assay was determined with various virus infected cell lines, normal human sera\/plasma, and serum from patients not known to be at risk for HIV infection. No false\u2010positive HIV\u2010Ag results were seen. Sera from 69% of patients with AIDS were positive for HIV\u2010Ag as were 46% of patients with ARC and 19% of asymptomatic, HIV\u2010antibody\u2010positive individuals. There were significant associations between the stage of HIV infection\u2013ie, AIDS vs ARC vs asymptomatic\u2010and the detection of HIV\u2010Ag in serum (p &lt; 0.0001) and the lack of detection of antibody to HIV core Ag (p &lt; 0.0001). HIV\u2010Ag was also found in the serum of two asymptomatic antibody\u2010negative individuals who were at high risk for AIDS and who later developed HIV antibody. The presence of HIV\u2010Ag in sera was confirmed by an inhibition procedure. Thus. HIV\u2010Ag can be detected in the serum of infected individuals prior to antibody production and correlates with the clinical stage of HIV infection.<\/jats:p>","DOI":"10.1002\/jmv.1890220408","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T13:28:31Z","timestamp":1172842111000},"page":"357-363","source":"Crossref","is-referenced-by-count":103,"title":["Correlation of serum HIV antigen and antibody with clinical status in HIV\u2010I infected patients"],"prefix":"10.1002","volume":"22","author":[{"given":"Deborah A.","family":"Paul","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence A.","family":"Falk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold A.","family":"Kessler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Chase","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Blaauw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Chudwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan L.","family":"Landay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6189183"},{"key":"e_1_2_1_3_1","first-page":"373","article-title":"Revision of the case definition of acquired immunodeficiency syndrome for national reporting\u2010United States","volume":"34","author":"Centers for Disease Control","year":"1985","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"e_1_2_1_4_1","first-page":"334","article-title":"Classification system for HTLV\u2010III\/LAV infections","volume":"35","author":"Centers for Disease Control","year":"1986","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3008335"},{"key":"e_1_2_1_6_1","first-page":"146","article-title":"A confirmatory assay for antibodies to HTLV III. International Conference on AIDS","volume":"642","author":"Dawson G","year":"1986","journal-title":"Abstract"},{"key":"e_1_2_1_7_1","unstructured":"FalkL PaulD LandayL KesslerH(1987): HIV isolation from plasma of HIV\u2010infected individuals. 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