{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T23:57:35Z","timestamp":1698105455821},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":6368,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1988,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A diagnostic hybridization assay for detecting herpes simplex virus type 1 (HSV\u20101) in ocular specimens was developed using cloned viral DNA as a probe. This hybridization assay is based on visualizing a biotinylated probe that is hybridized to the target DNA by a streptavidin\/alkaline phosphatase system. The time required for performing this assay system is only two days. This assay system could detect a probe which had been hybridized to as little as 1 pg of homologous DNA and did not cross\u2010react with DNA of other human herpes viruses except that of herpes simplex virus type 2 (HSV\u20102) which showed weak cross\u2010reactivity. The assay system was applied to experimental keratitis in albino rabbits and clinical specimens. In experimental keratitis in rabbits it was possible to detect HSV\u20101 DNA in the eye swab samples at least until the ninth day after virus inoculation. Five clinical specimens collected from patients with corneal ulcer or blepharitis contained HSV\u20101 DNA in spite of the failure of demonstration of viral antigen and\/or virus isolation in two cases.<\/jats:p>","DOI":"10.1002\/jmv.1890250303","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T16:21:27Z","timestamp":1172852487000},"page":"259-270","source":"Crossref","is-referenced-by-count":5,"title":["Detection of herpes simplex virus type 1 in herpetic ocular diseases by DNA\u2010DNA hybridization using a biotinylated DNA 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