{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T10:21:49Z","timestamp":1757586109612},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":6247,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1988,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oral \u201chairy\u201d leukoplakia (OHL) is a white lesion of the oral mucosa, usually located on the lateral tongue among human immunodeficiency virus (HIV) positive acquired immunodeficiency syndrome (AIDS)\u2010risk patients. The lesion has been reported to be associated with Epstein\u2010Barr virus (EBV) and human papillomavirus (HPV). Twenty surgical biopsy specimens were evaluated for the presence of HPV genus\u2010specific antigen, HPV 2\/4, 6\/11, and 16\/18 DNA and EBV DNA by in situ hybridization employing formalin\u2010fixed paraffin\u2010embedded sections. Three cases exhibited immunoreactivity for HPV genus\u2010specific antigen, with localization in cytopathically altered upper spinous layer keratinocytes. HPV 16, 18, or related DNA sequences were identifiable in a single case. Alternatively, employing an EBV long internal repeat subgenomic probe, 19 cases were found to harbor EBV DNA. In all positive cases, the hybrids were localized to upper spinous layer keratinocytes exhibiting nuclear\/cytoplasmic vesiculation. It is concluded that OHL is consistently associated with EBV; furthermore, viral replication, as evidenced by DNA localization, corresponds to ultrastructural evidence of capsid and envelope assembly in the more differentiated layers of oral epithelium.<\/jats:p>","DOI":"10.1002\/jmv.1890260307","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T16:24:54Z","timestamp":1172852694000},"page":"271-277","source":"Crossref","is-referenced-by-count":41,"title":["Detection of EBV and HPV DNA sequences in oral \u201chairy\u201d leukoplakia by in situ hybridization"],"prefix":"10.1002","volume":"26","author":[{"given":"L. R.","family":"Eversole","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. E.","family":"Stone","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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