{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:09:46Z","timestamp":1761487786752},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":5576,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1990,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Molluscum contagiosum lesions obtained from 75 Australian patients, 22 (29%) of whom were HIV positive, were examined by restriction endonuclease analysis and Southern blot hybridisation using radiolabelled and digoxigeninlabelled MCV DNA probes. The isolates were classified (MCV 1, 1v, 2, and 2v) on the basis of these results, which were in turn correlated with the clinical features of each lesion. A total of 44 (59%) of the lesions contained MCV 1 or 1v, 24 (32%) contained MCV 2 or 2v, three (4%) contained multiple MCV types, whereas four (5%) of lesions submitted contained no detectable MCV DNA. The ratio of MCV 1\/1v to MCV 2\/2v was determined to be approximately 1.75:1. There were substantial differences between the distribution of MCV types 1 and 2 among patients of different age groups, but no significant relationship between MCV type and the sex of the patient was found. MCV 2 was more frequently detected in lesions from anogenital areas and in immunosuppressed (HIV\u2010positive) patients, and MCV 1 was more commonly isolated from skin rather than genital lesions, but neither association was statistically significant. Fragment F (10 kbp) obtained from the genome of MCV 1 was capable of differentiating MCV 1 from MCV 2 when used as a probe in hybridisation experiments with BamHl cut samples and may be useful when small amounts of lesion prevents differentiation by direct visualisation of restriction endonuclease fragments.<\/jats:p>","DOI":"10.1002\/jmv.1890320102","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T18:51:28Z","timestamp":1172861488000},"page":"1-9","source":"Crossref","is-referenced-by-count":38,"title":["Molecular epidemiology of Australian isolates of molluscum contagiosum"],"prefix":"10.1002","volume":"32","author":[{"given":"Carol H.","family":"Thompson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita T.","family":"De Zwart\u2010Steffe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian M.","family":"Biggs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-198107000-00012"},{"key":"e_1_2_1_3_1","first-page":"507","volume-title":"Sexually Transmitted Diseases and Etiologic Agents","author":"Brown ST","year":"1984"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1987.tb04908.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890180105"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/151.2.376"},{"key":"e_1_2_1_7_1","first-page":"28","article-title":"Sexually transmitted diseases: Genital molluscum contagiosum","volume":"26","author":"Felman YM","year":"1980","journal-title":"Cutis"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1128\/jvi.19.2.382-388.1976","article-title":"Some biological and physical properties of molluscum contagiosum in cell culture","volume":"19","author":"Francis RD","year":"1976","journal-title":"Journal of Virology"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1971.tb14089.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1987.tb05801.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.121.7.834b"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(77)90141-6"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1001\/archderm.1978.01640150027008","article-title":"Atopic dermatitis, impaired cellular immunity and molluscum contagiosum","volume":"114","author":"Pauly CR","year":"1978","journal-title":"Archives of Dermatology"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800068072"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00039896.1970.10667262"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(85)80397-2"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.20.7207"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(85)80464-3"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/158.4.898"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890270203"},{"key":"e_1_2_1_21_1","first-page":"978","article-title":"Eruption molluscum contagiosum in atopic dermatitis","volume":"95","author":"Solomon LM","year":"1966","journal-title":"Journal of the Canadian Medical Association"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890320102","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890320102","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T20:07:25Z","timestamp":1698005245000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890320102"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,9]]}},"alternative-id":["10.1002\/jmv.1890320102"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890320102","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9]]}}}