{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:37:24Z","timestamp":1789364244047,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":5454,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of hepatitis B virus (HBV) genome in sera from 73 symptomatic and asymptomatic HBsAg carriers was studied by the polymerase chain reaction (PCR) with primers specific for the S and C regions. Pre\u2010S proteins of the HBV envelope were detected in serum by a specific monoclonal antibody in a double immunoradiometric assay.<\/jats:p><jats:p>Out of twenty\u2010five symptomatic patients with chronic active hepatitis (14 with HBeAg and 11 with anti\u2010HBe), all were positive for HBV DNA by PCR, while 14\/14 HBeAg and 2\/11 (18%) of the anti\u2010HBe patients were positive by dot blot hybridization. All but one anti\u2010HBe patient (96%) carried Pre\u2010S1 proteins. Among the asymptomatic HBsAg carriers, HBV DNA was detected by PCR in 14\/14 (100%) HBeAg positive patients and in 25\/34 (73%) anti\u2010HBe positive patients. Pre\u2010S1 proteins were found, respectively, in 14\/14 (100%) and 11\/22 (50%) of the same cases tested in parallel. The 20 healthy blood donors devoid of HBV markers and with normal transaminases tested were found negative for HBV DNA using PCR.<\/jats:p><jats:p>Out of 12 patients who recovered from acute hepatitis 6, all were found negative by PCR analysis after a mean follow up of 1 year after seroconversion to anti\u2010HBs. When serial samples from 2 patients (one with acute hepatitis 6, the other with chronic hepatitis 6) were tested for the presence of HBV DNA and of Pre\u2010S1 proteins, both markers showed parallel development.<\/jats:p><jats:p>The results indicate that HBV DNA detected by PCR is associated significantly with the presence of Pre\u2010S1 proteins (P &lt; 0.0001) and does reflect the presence of complete virions in sera from patients negative for HBV DNA by the conventional hybridization technique. The results also suggest a slower clearance of infectious particles than assumed previously.<\/jats:p>","DOI":"10.1002\/jmv.1890330111","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T19:01:24Z","timestamp":1172862084000},"page":"51-57","source":"Crossref","is-referenced-by-count":27,"title":["Correlation between HBV DNA detection by polymerase chain reaction and pre\u2010S1 antigenemia in symptomatic and asymptomatic hepatitis B virus infections"],"prefix":"10.1002","volume":"33","author":[{"given":"I.","family":"Chemin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Baginski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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