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Twenty\u2010one Colombian patients were tested, all of whom had antibodies to HTLV\u2010I; the presence of HTLV\u2010I proviral DNA in their PBMNC's was confirmed by the polymerase chain reaction technique. Of the 21 patients 15 had a clinical diagnosis of tropical spastic paraparesis (TSP\/HAM), 5 were asymptomatic relatives, and 1 patient had leukemia. In situ hybridization was positive in samples from 5 patients; 2 of these were TSP\/HAM patients and the other 3 were healthy wives of TSP\/HAM patients. This study demonstrates for the first time that viral RNA is expressed in uncultured PBMNC's of some patients with TSP\/HAM in whom proviral DNA is also present; furthermore, the detection of HTLV\u2010I RNA in the blood of female partners of TSP\/HAM patients clearly illustrates the high likelihood of HTLV\u2010I transmission through sexual contact.<\/jats:p>","DOI":"10.1002\/jmv.1890330113","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T19:01:24Z","timestamp":1172862084000},"page":"64-71","source":"Crossref","is-referenced-by-count":22,"title":["In situ hybridization detection of HTLV\u2010I RNA in peripheral blood mononuclear cells of TSP\/HAM patients and their spouses"],"prefix":"10.1002","volume":"33","author":[{"given":"M. A.","family":"Beilke","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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