{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T18:18:46Z","timestamp":1700417926727},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T00:00:00Z","timestamp":1134000000000},"content-version":"vor","delay-in-days":5396,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Degenerate oligonucleotide primers were designed for in vitro amplification by polymerase chain reaction (PCR) of a relatively well\u2010conserved portion of the L1 capsid protein gene of genital human papillomaviruses, A specific 441 bp fragment was amplified by PCR from genomic clones and clinical biopsy specimens containing DNAfrom HPV types6,11,16,18,31, and 33, as well as from a number of other clinical specimens known to contain unclassified HPV isolates. As some HPV non\u2010specific DNA was also often amplified, another set of degenerate primers was designed which amplified a 335 bp sequence contained within the 441 bp sequence. These nested primers could be used in a two\u2010stage PCR reaction to obtain distinct HPV\u2010spe\u2010cific DNA fragments suitable for direct sequencing. Two\u2010stage PCR was used to demonstrate the presence of HPV DNA in 13 out of 16 biopsies, which had been classified histologically as cervical intraepithelial neoplasia (CIN), but which were negative for HPV on Southern blot hybridization. Seven of these amplification products were sequenced, and one proved to be a previously unsequenced HPV type. The results have important implications for the routine detection and typing of genital and other HPV types in clinical samples.<\/jats:p>","DOI":"10.1002\/jmv.1890330305","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T19:21:06Z","timestamp":1172863266000},"page":"165-171","source":"Crossref","is-referenced-by-count":30,"title":["Detection of genital human papillomaviruses by polymerase chain reaction amplification with degenerate nested primers"],"prefix":"10.1002","volume":"33","author":[{"given":"Anna\u2010Lise","family":"Williamson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward P.","family":"Rybicki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.5.1309"},{"key":"e_1_2_1_3_1","first-page":"321","volume-title":"The Papovaviridae","author":"Baker CC","year":"1987"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890270207"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"issue":"12","key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/jcm.27.12.2660-2665.1989","article-title":"Amplification of human papillomavirus DNA sequences by using conserved primers","volume":"27","author":"Gregoire L","year":"1989","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_7_1","first-page":"1625","volume-title":"Virology","author":"Howley PM","year":"1990"},{"key":"e_1_2_1_8_1","first-page":"93","volume-title":"Molecular Cloning. 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