{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T08:47:47Z","timestamp":1698310067001},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":5273,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1991,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serum or plasma from 69 HBsAg\u2010positive patients was tested for the presence of precore\/core gene specific DNA by the polymerase chain reaction (PCR). In both healthy individuals (n = 26) and chronic carriers (n = 25), there was a strong correlation between presence of circulating anti\u2010HBe and the absence of detectable HBV genome in serum. In 18 serum samples where HBsAg was the only detectable marker, i.e., anti\u2010HBc\u2010negative specimens, HBV DNA could be detected in three samples. HBV strains from 21 of the 24 PCR\u2010positive samples were sequenced over the precore\/core junction. A stop codon at the end of the precore region, described by other workers, was found in strains from two blood donors, one of whom had detectable HBeAg in serum. Conversely, HBV strains from the three anti\u2010HBc\u2010negative patients where DNA of the HBV precore region could be amplified and who had no detectable serum HBeAg or anti\u2010HBe did not have this stop codon. The study indicates that further investigations are required before lack of HBeAg can be correlated with evidence of mutations in the precore region.<\/jats:p>","DOI":"10.1002\/jmv.1890340309","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T19:58:53Z","timestamp":1172865533000},"page":"179-183","source":"Crossref","is-referenced-by-count":22,"title":["Enzymatic amplification and sequence analysis of precore\/core DNA in HBsAg\u2010positive patients"],"prefix":"10.1002","volume":"34","author":[{"given":"Karin","family":"Ljunggren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alistair H.","family":"Kidd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890090109"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-8278(90)90062-V"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90713-7"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.13.4601"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.1.312"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(90)90971-3"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1128\/jvi.12.5.995-1005.1973","article-title":"DNA polymerase associated with the human hepatitis B virus","volume":"12","author":"Kaplan PM","year":"1973","journal-title":"Journal of Virology"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.14.5864"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1128\/jvi.64.3.1298-1303.1990","article-title":"Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen","volume":"64","author":"Okamoto H","year":"1990","journal-title":"Journal of Virology"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2448875"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890270409"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/317489a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(90)90030-U"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90598-F"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114326"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890340309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890340309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:02:30Z","timestamp":1698019350000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890340309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,7]]}},"alternative-id":["10.1002\/jmv.1890340309"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890340309","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7]]}}}