{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T04:52:44Z","timestamp":1698295964123},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4937,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The prevalence of anti\u2010HCV antibodies in Chinese patients with HBsAg\u2010negative chronic liver diseases was studied retrospectively. Anti\u2010HCV was detected by two different ELISAs. In 97 pa\u2010 tients with HBsAg\u2010negative chronic liver disease, 26 (27%) were anti\u2010HCV positive. Of 157 control subjects, only 1 (0.6%) was anti\u2010HCV positive <jats:italic>(P<\/jats:italic> &lt; 0.001). Anti\u2010HCV was detected in 18 of 27 (67%) patients with post\u2010transfusion non\u2010A, non\u2010B (PTNANB) chronic hepatitis or cirrhosis, 5 of 25 (20%) patients with cryptogenic chronic hepatitis or cirrhosis, 2 of 33 (6%) patients with alcoholic liver disease, 1 of 5 (20%) patients with autoimmune chronic active hepatitis (AICAH), none of 4 patients with primary biliary cirrhosis (PBC), and none of 3 patients with fatty liver. The prevalence in this group of patients was lower when compared to reports from other countries. The addition of a urea washing step reduced false\u2010positivity in alcoholic and AICAH groups. The ELISA that employs three recombinant HCV antigens confirmed all positive results by an\u2010 other ELISA with the exception of one weakly positive result in the AICAH group and one in the alcoholic group. One patient in the PTNANB group was detected in addition by the second generation assay. In conclusion, ELISA with a urea wash proved to be useful in reducing falsepositivity, and the second generation assay proved to be a sensitive and specific test for anti\u2010HCV antibody. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890370215","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T21:28:05Z","timestamp":1172870885000},"page":"158-160","source":"Crossref","is-referenced-by-count":7,"title":["Prevalence of antibody to hepatitis C virus in HBsAg\u2010negative chronic liver disease in Hong Kong using different assays"],"prefix":"10.1002","volume":"37","author":[{"given":"J. Y.","family":"Lai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John S.","family":"Tam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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