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Enteroviral RNA was detected in faeces of all 36 patients in whom an enterovirus was isolated from stool. The PCR assay yielded positive results in additionally 316 cases where enterovirus diagnoses were obtained by virus isolation from cerebrospinal fluid and\/or serological tests. Thus, the positive outcome of the PCR assay was 39 (93%) among the 42 patients with enterovirus diagnoses. Furthermore, 7\/19 (37%) cases with an etiology that was not established by other means were positive in the test indicating that the PCR assay may give considerable additional etiological information in patients with aseptic meningitis. The limit of RNA detectability in the PCR assay was about 100 TCID, when highly cytopathogenic enterovirus types (coxsackievirus type B5 and echovirus type 11) were tested.<\/jats:p><jats:p>The PCR was negative in all 13 patients with non\u2010enterovirus diagnoses except in one case with a herpes simplex virus type 2 infection. Since enterovirus\u2010specific IgM antibodies could be detected in this case a dual infection seemed probable. All the negative controls, included in the study, were PCR\u2010negative and no contamination was encountered.<\/jats:p><jats:p>This study proves the usefulness of the PCR assay for detection of enteroviral RNA in stool samples and suggests that the test may be an alternative to virus isolation for rapid enterovirus diagnosis in patients with aseptic meningitis. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890380112","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T21:35:39Z","timestamp":1172871339000},"page":"54-61","source":"Crossref","is-referenced-by-count":35,"title":["Detection of enteroviral RNA by polymerase chain reaction in faecal samples from patients with aseptic meningitis"],"prefix":"10.1002","volume":"38","author":[{"given":"Martin","family":"Glim\u00e5ker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Almaz","family":"Abebe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anneka","family":"Ehrnst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Per","family":"Olc\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orjan","family":"Stranneg\u00e5rd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"AbebeA(1991):Application of the polymerase chain reaction for the ' detection of enterovirus in clinical samples. 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