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One assay was based on heat\u2010treated virions (H\u2010EIA) and one on synthetic peptides (PEIA) as antigens. The sensitivities, with virus isolation as reference method, were 0.67 by H\u2010EIA and 0.62 by P\u2010EIA, which were higher than by a routinely used complement fixation test (CFT, 0.51) but somewhat lower than the sensitivities found by two previously presented IgM\u2010assays, \u03bc\u2010capture EIA, and solid\u2010phase reverse immunosorbent test (SPRIST). The specificities of the two IgG\u2010EIA techniques and CFT were apparently high, whereas the two IgM\u2010assays showed positive reactions in some non\u2010enteroviral cases. A relatively rapid increase in enteroviral IgG\u2010antibodies was apparent using H\u2010EIA and P\u2010EIA. The two IgG\u2010EIA tests contributed with considerable additional etiological information since significant IgG\u2010rises were obtained in 13 patients by H\u2010EIA and in 19 by P\u2010EIA, respectively, out of the 56 individuals in whom enterovirus isolation was negative and a non\u2010enteroviral diagnosis was not found. Thus, detection of enteroviral IgG\u2010antibodies by H\u2010EIA and P\u2010EIA seems to be a valuable alternative to CFT for the routine diagnosis of enteroviral meningitis. The IgM\u2010assays, \u03bc\u2010capture EIA, and SPRIST, may allow a relatively rapid report of an enteroviral infection. However, since both the IgM\u2010tests are hampered by incomplete specificities, a confirmation of positive results by an IgG\u2010assay should be carried out. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890400404","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T22:38:43Z","timestamp":1172875123000},"page":"271-277","source":"Crossref","is-referenced-by-count":19,"title":["Diagnosis of enteroviral meningitis with IgG\u2010EIA using heat\u2010treated virions and synthetic peptides as 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