{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T08:42:17Z","timestamp":1698309737158},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4480,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A multi\u2010targeted \u201chemi\u2010nested\u201d PCR (M\u2010PCR) assay in which the primer pairs derived from the 5\u2032 non\u2010coding (5\u2032NC) and the nonstructural protein 3 (NS3) regions of HCV genome were concurrently used for amplification in order to compare the sensitivity and specificity of polymerase chain reaction (PCR) with different primer pairs in detecting hepatitis C virus (HCV) genome. Sera from patients with virus\u2010associated liver diseases were examined for the presence of HCV RNA by the M\u2010PCR method following reverse transcription to cDNA. The amplified products derived from both the 5\u2032NC and the NS3 regions were detected in 28 (70%) of the 40 HCV RNA\u2010positive samples. However, 12 samples (30%) were devoid of the signal of NS3\u2010derived product. Sensitivity tests using serial dilutions of HCV RNA revealed that the 5\u2032NC\u2010derived band was still detectable in the 10<jats:sup>5<\/jats:sup>\u2010fold diluted sample by the M\u2010PCR method, yet the NS3\u2010derived band could hardly be detected in the 10<jats:sup>4<\/jats:sup>\u2010fold diluted sample. Thus, as previously demonstrated by a single\u2010targeted \u201cnested\u201d PCR assay, the present study using the M\u2010PCR assay has clearly shown that the 5\u2032NC\u2010derived primers are more sensitive and specific than the NS3\u2010derived primers in detecting HCV RNA.<\/jats:p>","DOI":"10.1002\/jmv.1890410103","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T23:04:48Z","timestamp":1172876688000},"page":"6-10","source":"Crossref","is-referenced-by-count":6,"title":["Detection of hepatitis C virus genome in human serum by multi\u2010targeted polymerase chain reaction"],"prefix":"10.1002","volume":"41","author":[{"given":"M.","family":"Okamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Baba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Kodama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Sekine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Takagi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Kasukawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Shigeta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-7041-3_8"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198911303212202"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890340213"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.1.187"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2523562"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.6.2451"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90269-H"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)91446-H"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840140227"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840140404"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.22.10292"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)91042-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.24.10367"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2496467"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.3.983"},{"key":"e_1_2_1_17_1","first-page":"167","article-title":"The 5\u2032\u2010 terminal sequence of the hepatitis C virus genome","volume":"60","author":"Okamoto H","year":"1990","journal-title":"Japanese Journal of Experimental Medicine"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-72-11-2697"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.16.6441"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90134-Q"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890350409"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890410103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890410103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:35:16Z","timestamp":1698212116000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890410103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,9]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,9]]}},"alternative-id":["10.1002\/jmv.1890410103"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890410103","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,9]]}}}