{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:01:08Z","timestamp":1790517668611,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4480,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two hundred and twelve urine specimens, from several clinical groups, were examined for BK virus (BKV) using the polymerase chain reaction (PCR) to detect the VP1 region of BKV DNA. Positive results were obtained on 14 specimens from 44 post\u2010transplant patients (31.8%), 10 specimens from 39 pregnant women (25.6%), and 5 specimens from 100 children (5%) but not on any specimens from 29 laboratory staff. Twentyeight of the amplified BKV genomes, 19 from urine specimens, eight from culture fluid of inoculated tissue, and also one from a throat washing were directly sequenced from single\u2010stranded templates immobilized via a biotinylated primer; it was possible to assign all to one of the four subtypes of BKV which had previously been identified on the basis of variation in nucleotide sequence of the VP1 region. Serological subgroup classification correlated with the genomic subtyping results in 21 of the isolates. The distribution of the BKV subtypes and the clinical status of the infected individuals are discussed.<\/jats:p>","DOI":"10.1002\/jmv.1890410104","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T23:04:48Z","timestamp":1172876688000},"page":"11-17","source":"Crossref","is-referenced-by-count":128,"title":["Genomic typing of BK virus in clinical specimens by direct sequencing of polymerase chain reaction products"],"prefix":"10.1002","volume":"41","author":[{"given":"Li","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia E.","family":"Gibson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Booth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan P.","family":"Clewley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198701000-00024"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890160105"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1128\/jcm.27.6.1174-1179.1989","article-title":"Detection of BK virus and JC virus in urine and brain tissue by the polymerase chain reaction","volume":"27","author":"Arthur RR","year":"1989","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/158.3.563"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"3190","DOI":"10.1128\/jvi.61.10.3190-3198.1987","article-title":"Missense mutations in the VP1 gene of Simian virus 40 that compensate for defects caused by deletions in the viral agnogene","volume":"61","author":"Barkan A","year":"1987","journal-title":"Journal of Virology"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.31.4.338"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/142.1.1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910390111"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/158.6.1177"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1128\/jvi.65.1.342-349.1991","article-title":"Polyomavirus replication in mice: Influences of VP1 type and route of inoculation","volume":"65","author":"Dubensky TW","year":"1991","journal-title":"Journal of Virology"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.21.10393"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(87)90071-7"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1128\/jvi.65.1.335-341.1991","article-title":"Polyomavirus tumor induction in mice: Effects of polymorphisms of VP1 and large T antigens","volume":"65","author":"Freund R","year":"1991","journal-title":"Journal of Virology"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1128\/jvi.65.1.350-355.1991","article-title":"A single\u2010aminoacid substitution in polyomavirus VP1 correlates with plaque size and haemagglutination behaviour","volume":"65","author":"Freund R","year":"1991","journal-title":"Journal of Virology"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.51.2.458-469.1984"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)91776-4"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.37.5.578"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890100203"},{"key":"e_1_2_1_20_1","first-page":"216","article-title":"Mutations induced during the polymerase chain reaction","volume":"2","author":"Hayashi K","year":"1990","journal-title":"Technique"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-92-3-373"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890390110"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890280212"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"4515","DOI":"10.1128\/jvi.65.8.4515-4519.1991","article-title":"BK virus and JC virus shed during pregnancy have predominantly archetypal regulatory regions","volume":"65","author":"Markowitz R\u2010B","year":"1991","journal-title":"Journal of Virology"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0890-8508(91)90006-6"},{"key":"e_1_2_1_26_1","first-page":"479","volume-title":"Principles and Practice of Clinical Virology","author":"McCance DJ","year":"1987"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1128\/jvi.59.2.500-505.1986","article-title":"Molecular and biological properties of BK virus\u2010IR, a BK virus variant isolated from a human tumor","volume":"59","author":"Pagnani M","year":"1986","journal-title":"Journal of Virology"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(83)90509-3"},{"key":"e_1_2_1_29_1","doi-asserted-by":"crossref","first-page":"1600","DOI":"10.1128\/jvi.65.3.1600-1604.1991","article-title":"Recurring theme of changes in the transcriptional control region of BK virus during adaptation to cell culture","volume":"61","author":"Rubinstein R","year":"1991","journal-title":"Journal of Virology"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(79)90209-5"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/128.6.784"},{"key":"e_1_2_1_33_1","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1128\/jvi.63.2.901-911.1989","article-title":"Nucleotide sequence of the human polyomavirus AS virus, an antigenic variant of BK virus","volume":"63","author":"Tavis JE","year":"1989","journal-title":"Journal of Virology"},{"key":"e_1_2_1_34_1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/jvi.59.2.260-266.1986","article-title":"Evolutionary changes of transcriptional control region in a minute\u2010plaque viable deletion mutant of BK virus","volume":"59","author":"Watanabe S","year":"1986","journal-title":"Journal of Virology"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890410104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890410104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:35:19Z","timestamp":1698212119000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890410104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,9]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,9]]}},"alternative-id":["10.1002\/jmv.1890410104"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890410104","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,9]]}}}