{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:18:13Z","timestamp":1788347893348,"version":"build-2803163510"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4480,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis A virus infections have been reported recently among hemophilic patients in Italy and Germany, leading to speculation that infectious hepatitis A virus (HAV) might have been present in some factor VIII concentrates. In both cases, the implicated factor concentrates had been treated by a solvent\/detergent method, which inactivates enveloped viruses but which would not be expected to inactivate HAV, a nonenveloped picornavirus. To determine whether HAV would be inactivated during pasteurization of factor VIII concentrate, an alternative method employed for virus inactivation, we determined the extent to which the infectivity of cell culture\u2010adapted HAV, suspended either in cell culture medium or in a proprietary stabilizing buffer, was reduced by heat treatment at 60\u00b0C for 10 hr. The titer of infectious HAV declined rapidly at 60\u00b0C, but the stabilizer considerably delayed HAV inactivation. In cell culture medium, HAV was inactivated by &gt;3.6 log<jats:sub>10<\/jats:sub> within 30 min, but 3.6 log<jats:sub>10<\/jats:sub> inactivation of HAV was reached only after 6 hr in the presence of the stabilizer. Residual infectious HAV was present after even 10 hr of heat treatment in the stabilizer, indicating that &lt;5.2 log<jats:sub>10<\/jats:sub> infectious HAV particles are inactivated under these conditions. In the presence of the stabilizer, HAV was significantly more stable than poliovirus type 1, which has been used to validate virus inactivation by pasteurization. We conclude that pasteurized factor VIII concentrate should pose little if any risk for transmission of HAV if pooled plasma used for its manufacture contained low levels of the virus.<\/jats:p>","DOI":"10.1002\/jmv.1890410113","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T23:04:48Z","timestamp":1172876688000},"page":"61-64","source":"Crossref","is-referenced-by-count":43,"title":["Inactivation of hepatitis a virus by heat treatment in aqueous solution"],"prefix":"10.1002","volume":"41","author":[{"given":"Paula","family":"Murphy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Nowak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley M.","family":"Lemon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Hilfenhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/1045-1056(91)90044-K"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92937-B"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1986.tb04882.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1983.03340170039025"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(62)90161-4"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM195910082611501"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.1.7"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890100105"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1128\/jvi.65.4.2056-2065.1991","article-title":"Antigenic and genetic variation of cytopathic hepatitis A virus variants arising during persistent infection: Evidence for genetic recombination","volume":"65","author":"Lemon SM","year":"1991","journal-title":"Journal of Virology"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)91955-8"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92938-C"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890360311"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1001\/archinte.1984.00350200071011","article-title":"Transmission of hepatitis A by transfusion of blood products","volume":"144","author":"Sheretz RJ","year":"1984","journal-title":"Archives of Internal Medicine"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149554"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149554"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.4.647"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890410113","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890410113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:34:58Z","timestamp":1698212098000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890410113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,9]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,9]]}},"alternative-id":["10.1002\/jmv.1890410113"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890410113","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,9]]}}}