{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T05:10:19Z","timestamp":1698297019103},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4389,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate clonality of local T cells in viral infections of the central nervous system, the T\u2010cell receptor (TCR) repertoire was evaluated in T cells from the cerebrospinal fluid (CSF) of nine patients with mumps meningitis, using the polymerase chain reaction (PCR) assay. The usage of the variable region of TCR \u03b1 chain gene (V\u03b1 gene) in the CSF was widespread, and an average of 13 out of the 18 V\u03b1 families were expressed. Quantitative PCR analyses showed that the V\u03b1 gene expression was biased toward three or less V\u03b1 families in the CSF of each patient. When compared with peripheral blood T cells, the average percentages of V\u03b1 11 and V\u03b1 12 gene expression were significantly higher in the CSF than in the peripheral blood. These results suggested that mumps\u2010specific T lymphocytes with a restricted TCR repertoire are selectively recruited to the central nervous system in mumps meningitis, although polyclonal, probably nonspecific, T\u2010cell populations are present in the CSF.<\/jats:p>","DOI":"10.1002\/jmv.1890410409","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T23:25:07Z","timestamp":1172877907000},"page":"306-311","source":"Crossref","is-referenced-by-count":3,"title":["T\u2010cell receptor V\u03b1 region usage in the cerebrospinal fluid of patients with mumps 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