{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T10:52:24Z","timestamp":1700391144101},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4238,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mutations in the precore region of hepatitis B virus (HBV) have been associated with failure of expression of HBV e\u2010antigen (HBeAg), however, the prevalence of these and other mutations in HBV carriers without overt chronic liver disease remains uncertain. Homosexual or bisexual males (n = 65) with chronic HBV infection attending The Middlesex Hospital, London were studied, of whom two had clinical evidence of chronic liver disease. HBV DMA was amplified from 62 of 65 serum samples using nested and double nested polymerase chain reaction (PCR) assays. Direct sequencing of the PCR products was employed to investigate sequence variation. HBV\u2010DNA from all available HBeAg\u2010negative (n = 9) and selected HBeAg\u2010positive (n = 33) sera were sequenced in the entire precore gene, the 3\u2032 terminal portion of the X gene (aa128\u2013154), and the 5\u2032 terminus of the core gene (aa18\u201373). Sequences were highly conserved in all regions studied. Samples from two anti\u2010HBe\u2010seropositive patients contained mutations in the precore region. In one, a single mutation in the first amino acid resulted in a change to leucine, which would prevent translation of this region and therefore HBeAg expression. Wild type sequences were also detected in this sample. In the other sample from a patient with overt chronic liver disease, a mutation of precore amino acid 28 changed a tryptophan residue to a stop codon which would also prevent HBeAg expression. Although few such patients were studied, precore mutations may be uncommon in anti\u2010HBe\u2010seropositive patients without overt chronic liver disease. Possibly such mutations are not related to HBeAg to anti\u2010HBe seroconversion, but rather they may arise in patients who remain anti\u2010HBe seropositive for prolonged periods and they may be causally associated with the development of chronic liver disease. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890430103","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T00:03:30Z","timestamp":1172880210000},"page":"5-12","source":"Crossref","is-referenced-by-count":13,"title":["Conservation of precore and core sequences of hepatitis B virus in chronic viral carriers"],"prefix":"10.1002","volume":"43","author":[{"given":"A. E.","family":"Hawkins","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J. C.","family":"Gilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. A.","family":"Bickerton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S.","family":"Tedder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. V. D.","family":"Weller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(90)90632-B"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0950-3528(90)90059-P"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.10.4186"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90713-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199102000-00014"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840070424"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199106133242405"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890340309"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.25.11.1283"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.5.2941"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)90101-4"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"1298","DOI":"10.1128\/jvi.64.3.1298-1303.1990","article-title":"Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen","volume":"64","author":"Okamoto H","year":"1990","journal-title":"Journal of Virology"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199106133242404"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.6.1747"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890200305"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)91022-9"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.64.12.5840-5850.1990"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1128\/jvi.64.2.864-872.1990","article-title":"Human immunodeficiency virus\u2010infected individuals contain provirus in small numbers of peripheral mononuclear cells","volume":"64","author":"Simmonds P","year":"1990","journal-title":"Journal of Virology"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890060407"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/317489a0"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890320208"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.26.7.745"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.3.1266"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890430103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890430103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T01:21:00Z","timestamp":1698196860000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890430103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5]]},"references-count":24,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,5]]}},"alternative-id":["10.1002\/jmv.1890430103"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890430103","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5]]}}}