{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T12:30:49Z","timestamp":1783859449526,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":4238,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1994,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>JC virus (JCV), the causative agent of a human demyelinating disease, progressive multifocal leukoencephalopathy, has a very narrow host range. Cells permissive for infection by JCV have been essentially limited to primary human fetal glial cells, which are difficult to obtain and maintain. In pilot studies, it was found that JCV can multiply in an established cell line of human neuroblastoma. JCV strains Mad\u20101 and Tokyo\u20101 were inoculated, respectively, into two cell lines, IMR\u201032 (neuroblastoma) and A\u2010172 (glioblastoma). Viral infection with cytopathic effect was observed only in IMR\u201032 cells, and the most efficient viral proliferation was obtained in cells cultured in medium containing 2% fetal calf serum (FCS). Both Mad\u20101 and Tokyo\u20101 strains propagated well, with the former being more efficient than the latter. Viral replication was confirmed by immunofluorescence, electron microscopy, and a hemagglutination assay. Sodium dodecyl sulfate (SDS)\u2010polyacrylamide gel electrophore\u2010sis and Western blot analysis of the purified virus revealed the characteristic JCV protein profile. Thus, IMR\u201032 cells have been found to be permissive for JCV, which should provide a useful system for further studies of virus proliferation and viral tissue tropism. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890430104","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T00:03:30Z","timestamp":1172880210000},"page":"13-19","source":"Crossref","is-referenced-by-count":26,"title":["Propagation of JC virus in human neuroblastoma cell line IMR\u201032"],"prefix":"10.1002","volume":"43","author":[{"given":"Kaoru","family":"Akatani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayumi","family":"Imai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michio","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Nagashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuko","family":"Ikegami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1128\/jvi.65.2.1002-1006.1991","article-title":"Human fetal Schwann cells support JC virus multiplication","volume":"65","author":"Assouline 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