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To determine the capacity for removal or inactivation of hepatitis A virus during the factor VIM manufacturing process, human plasma and various factor VIM production intermediates were spiked with cell culture\u2010propagated virus and subjected to scaled down conditions mimicking the manufacture of solvent\/ detergent factor VIII. The combination of antibody\u2010mediated neutralization, cryoprecipitation, anion exchange chromatography, and lyophilization in the absence of sucrose resulted in a minimal reduction of 5.5 to 8.55 Iog<jats:sub>10<\/jats:sub> in the infectivity of hepatitis A virus. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890430109","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T00:03:30Z","timestamp":1172880210000},"page":"44-49","source":"Crossref","is-referenced-by-count":32,"title":["Removal\/neutralization of hepatitis A virus during manufacture of high purity, solvent\/detergent factor VIII concentrate"],"prefix":"10.1002","volume":"43","author":[{"given":"Stanley M.","family":"Lemon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula 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