{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T13:19:00Z","timestamp":1773494340557,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":3934,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1995,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Serum samples from 316 patients visiting the Dutch National Hemophilia Center were collected from 1979 to 1993 and stored at \u221230\u00b0C. Patients were placed into three different groups: (1) patients ever treated with large pool non\u2010hepatitis C virus (HCV)\u2010safe concentrate (n=179); (2) patients treated with cryoprecipitate (n = 125); and (3) patients treated exclusively with HCV\u2010save concentrate (n=12). In order to examine the prevalence of HCV infection in the different treatment groups serum samples were tested retrospectively for anti\u2010HCV antibody using second generation enzyme\u2010linked immunosorbent assay (ELISA) and recombinant immunoblot assay (RIBA\u20102). Significant differences in the prevalence of HCV infection were found between these 3 groups (group 1: 99%, group 2: 66%, group 3: 0%). The safety of currently administered clotting products is demonstrated in 57 patients who remained without HCV markers between 1989 and 1993. To examine the natural course of HCV infection fresh\u2010frozen plasma samples were obtained recently from a subgroup of 277 hemophilia patients for HCV\u2010RNA detection by a well\u2010validated cDNA\u2010PCR assay. In contrast to other reports, no evidence was found for seronegative HCV carriers. None of 52 patients without anti\u2010HCV had detectable HCV\u2010RNA. Of 225 patients with anti\u2010HCV, 182 (81%) were HCV\u2010RNA positive. None of 39 anti\u2010HCV positive patients with a negative HCV\u2010RNA reaction had serum alanine aminotransferase (ALT) levels above 50 U\/l, whereas 44% of HCV\u2010RNA positive patients had persistently elevated ALT levels above 50 U\/l. These results indicate that 20% of hemophilia patients who have been infected with HCV in the past eliminated the virus or have viral replication below the detection limit of polymerase chain reaction (PCR) without biochemical evidence of liver damage. \u00a9 1995 Wiley\u2010Liss, inc.<\/jats:p>","DOI":"10.1002\/jmv.1890450302","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T00:37:31Z","timestamp":1172882251000},"page":"241-246","source":"Crossref","is-referenced-by-count":56,"title":["Hepatitis C infection and viremia in Dutch Hemophilia patients"],"prefix":"10.1002","volume":"45","author":[{"given":"E. P.","family":"Mauser\u2010Bunschoten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Roosendaal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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