{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T17:49:56Z","timestamp":1772041796467,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":3812,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1995,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Detection of cytomegalovirus (CMV) DNA by the polymerase chain reaction (PCR) in samples of cerebrospinal fluid (CSF) has been shown to be a sensitive method of diagnosing CMV disease in the central nervous system. Since CMV causes latent infection in white blood cells, an unanswered question is whether detection of latent CMV DNA in the cell fraction of CSF samples by PCR is possible in seropositive patients. In a prospective study, the finding of CMV DNA in CSF of CMV seropositive patients with suspected viral infection of the central nervous system (CNS) was evaluated clinically. Fractionation of 64 CSF samples from seropositive patients was carried out before analysing the samples for CMV DNA by PCR. In four of the five patients who had CMV DNA in the cell pellet and\/or supernatant, the clinical data suggested CMV\u2010associated neurological disease. The remaining 59 samples were negative in both pellet and supernatant. In addition, 11 CSF samples with high cell counts from patients with bacterial meningitis were examined for CMV DNA and found to be negative in 10 patients and positive in 1. One hundred thirty two uncentrifuged CSF samples were used as negative controls. The results of the study indicate that detection of CMV DNA in CSF samples by PCR correlated well with disease and was not due to latent CMV infection. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890460319","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T00:53:54Z","timestamp":1172883234000},"page":"274-280","source":"Crossref","is-referenced-by-count":32,"title":["Detection of cytomegalovirus DNA in cerebrospinal fluid in immunocompetent patients as a sign of active infection"],"prefix":"10.1002","volume":"46","author":[{"given":"M.","family":"Studahl","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Bergstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Ekeland\u2010Sj\u00f6berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Ricksten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(85)90242-4"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92155-U"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.6.1408"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.43.1_Part_1.75"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1073046"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(91)90125-J"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.23.2.136"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.6.1416"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/978-1-349-20235-5_4","volume-title":"PCR Technology: Principles and Application for DNA Amplification","author":"Higuchi R","year":"1989"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.2.289"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1128\/jcm.12.1.46-51.1980","article-title":"Solid phase enzyme immunoassay for determination of antibodies to cytomegalovirus","volume":"12","author":"Krishna RV","year":"1980","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/339237a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410350522"},{"key":"e_1_2_1_15_1","first-page":"65","volume-title":"Structural Proteins: A Report of the First Nomenclature Workshop","author":"Landini MP","year":"1993"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(87)80012-6"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.1994.tb01262.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.1991.tb04903.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-198611000-00003"},{"key":"e_1_2_1_20_1","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.1001\/jama.1977.03280220067027","article-title":"Cytomegalovirus encephalitis in immunologically normal adults","volume":"238","author":"Phillips CA","year":"1977","journal-title":"Journal of American Medical Association"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890320202"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1128\/jcm.29.7.1513-1516.1991","article-title":"Detection of varicella\u2010zoster virus DNA by polymerase chain reaction in the cerebrospinal fluid of patients suffering from neurological complications associated with chicken pox or herpes zoster","volume":"29","author":"Puchhammer\u2010St\u00f6ck1 E","year":"1991","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_1_23_1","volume-title":"Molecular Cloning, A Laboratory Manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00862974"},{"key":"e_1_2_1_25_1","unstructured":"Singer\u2010SamJ TanguayRL RiggsAD(1991): Use of Chelex to irnprove the PCR signal from a small number of cells. 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