{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T07:02:16Z","timestamp":1765609336131},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,14]],"date-time":"2005-12-14T00:00:00Z","timestamp":1134518400000},"content-version":"vor","delay-in-days":3757,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of this study was to examine the efficacy of semi\u2010quantitative polymerase chain reaction (<jats:italic>PCR<\/jats:italic>) testing in cervical smears as an adjunct to cytological surveillance in a cohort of women with mild or moderate dyskaryosis. The study population comprised a group of 62 women who underwent twelve months of cytological and col\u2010poscopic surveillance as part of a larger randomised prospective study of women with mild and moderate dyskaryosis. Semi\u2010quantitative PCR for HPV\u201016 DNA was carried out on the initial and twelve month study smears before a large loop excision of the transformation zone (LLETZ) was carried out. Smears from a control population which comprised 167 women without a history of abnormal cervical cytology who were attending family planning and general practitioner clinics for routine cervical smears were tested similarly.<\/jats:p><jats:p>The presence of high or intermediate levels of HPV\u201016 DNA on both the initial and twelve month study smear was positively associated with the identification of cervical intraepithelial neoplasia (CIN) grades II or III in the LLETZ specimen (P=0.01). While the combination of HPV\u201016 DNA testing with cytology on a repeat cervical smear improved the selection of women with underlying CIN II\/III, there was still a false negative rate of 53%. Twenty\u2010nine women had \u2018low risk\u2019 levels of HPV\u201016 DNA and mild dyskaryosis or less on both repeat smears indicating suitability for surveillance, but in fact 34% of them had CIN II\/III.<\/jats:p><jats:p>This study supports the finding reported previously of an association between high and intermediate levels of HPV\u201016 DNA and CIN II\/III. This suggests that semi\u2010quantitative PCR may be a useful adjunct to cytology for selecting those women with mildly abnormal smears who would benefit most from colposcopy. A significant fluctuation in the levels of HPV\u201016 DNA was not detected when repeated smears were studied, suggesting that serial estimations of HPV\u201016 levels would add little to cytological examination for the subsequent surveillance of women considered to be at low risk of CIN II\/III. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890470103","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T02:40:43Z","timestamp":1172889643000},"page":"6-9","source":"Crossref","is-referenced-by-count":14,"title":["Serial quantitation of HPV\u201016 in the smears of women with mild and moderate dyskaryosis"],"prefix":"10.1002","volume":"47","author":[{"given":"G.","family":"Flannelly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Melvin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Mann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry","family":"Kitchener","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0957-5847(91)90060-B"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1993.110"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1001\/jama.1991.03460040048027","article-title":"Genital human papillomavirus infection in female university students as determined by a PCR based method","volume":"265","author":"Bauer HM","year":"1991","journal-title":"Journal of American Medical Association"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)91532-D"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1989.tb03382.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.308.6941.1399"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.301.6753.641"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1991.tb15344.x"},{"key":"e_1_2_1_10_1","first-page":"209","volume-title":"Cancer Cells: Molecular Diagnostics of Human Cancer","author":"Manos MM","year":"1989"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)92807-2"},{"key":"e_1_2_1_12_1","first-page":"9.16","volume-title":"Molecular cloning: A laboratory manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.298.6665.14"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890470103","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890470103","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T23:58:21Z","timestamp":1698278301000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890470103"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,9]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,9]]}},"alternative-id":["10.1002\/jmv.1890470103"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890470103","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,9]]}}}